AKTide-2T

Product Name:AKTide-2T

CAS No:324029-01-8

Purity:95%

Molar Mass:1715.89

Chemical Formula:C74H114N28O20

Storage:Store at -20 degrees Celsius

Sequence:ARKRERTYSFGHHA

Target: Akt/PKB

Application:

AKTide-2T is a synthetic peptide substrate specifically designed for studying the activity of protein kinase B (AKT). This peptide mimics the phosphorylation site of AKT substrates, making it a useful tool for monitoring AKT kinase activity in various biochemical assays. AKT plays a crucial role in regulating cellular processes such as metabolism, proliferation, and survival, making AKTide-2T essential in research exploring cancer, diabetes, and other disorders related to dysregulated AKT signaling. Its reliable performance in in vitro kinase assays enables precise analysis of AKT-mediated phosphorylation events, aiding in the development of potential therapeutic interventions.

Current Research:

AKTide-2T is a synthetic peptide substrate specifically designed for the serine/threonine kinase Akt, also known as Protein Kinase B (PKB). This peptide emulates the optimal phosphorylation motif recognized by Akt, rendering it a valuable tool for in vitro kinase assays. Notably, AKTide-2T exhibits competitive inhibition of histone H2B phosphorylation, with a reported inhibition constant (K?) of 12 nM .
The sequence of AKTide-2T is Ala-Arg-Lys-Arg-Glu-Arg-Thr-Tyr-Ser-Phe-Gly-His-His-Ala (ARKRERTYSFGHHA), which closely aligns with the consensus phosphorylation sequence preferred by Akt. This design enhances its efficacy as a substrate in kinase assays, facilitating the study of Akt activity and its modulation by potential inhibitors or activators.
In the context of kinase activity assays, AKTide-2T serves as a reliable substrate for evaluating the catalytic function of Akt isoforms. Its application extends to the assessment of Akt-mediated phosphorylation events, thereby contributing to a deeper understanding of Akt signaling pathways. Moreover, AKTide-2T has been utilized in the development of immunoassays aimed at quantifying Akt activity. For instance, certain assay kits employ plates pre-coated with AKTide-2T to capture phosphorylated substrates, which are subsequently detected using phospho-specific antibodies .
The specificity and high affinity of AKTide-2T for Akt make it an indispensable tool in biochemical studies focused on Akt signaling. Its use facilitates the elucidation of Akt's role in various cellular processes, including cell growth, survival, and metabolism. Furthermore, AKTide-2T aids in the screening and characterization of pharmacological agents targeting Akt, thereby advancing therapeutic strategies for diseases where Akt signaling is dysregulated.
In summary, AKTide-2T is a meticulously engineered peptide substrate that significantly contributes to the study of Akt kinase activity. Its application in research enhances our comprehension of Akt-mediated signaling pathways and supports the development of targeted therapeutic interventions.

Reference:

Karege, F., Perroud, N., Burkhardt, S., Schwald, M., Ballmann, E., La Harpe, R., & Malafosse, A. (2007). Alteration in kinase activity but not in protein levels of protein kinase B and glycogen synthase kinase-3?? in ventral prefrontal cortex of depressed suicide victims. Biological psychiatry, 61(2), 240-245.