AUTOCAMTIDE-2

Product Name:AUTOCAMTIDE-2

Synonyms:L-Lysyl-L-lysyl-L-alanyl-L-leucyl-L-arginyl-L-arginyl-L-glutaminyl-L-glutamyl-L-threonyl-L-valyl-L-aspartyl-L-alanyl-L-leucine (ACI)

CAS No:129198-88-5

Purity:95%

Molar Mass:1527.77

Chemical Formula:C65H118N22O20

Storage:Store at -20 degrees Celsius

Sequence:KKALRRQETVDAL

Target:calcium/calmodulin-dependent protein kinase II

Application:

Autocamtide-2 is a synthetic peptide substrate specifically designed for the study of Ca2?/calmodulin-dependent protein kinase II (CaMKII) activity. This peptide mimics the autophosphorylation site of CaMKII, making it a valuable tool for investigating the enzyme's regulatory mechanisms and its role in cellular signaling pathways. Autocamtide-2 is frequently used in biochemical assays to measure CaMKII activity and to understand its involvement in various physiological processes, including memory formation, synaptic plasticity, and cardiac function. Its high specificity and reliability make it ideal for use in research exploring the therapeutic potential of modulating CaMKII activity in neurological and cardiovascular disorders.

Current Research:

Autocamtide-2 is a synthetic peptide widely used as a substrate for the calcium/calmodulin-dependent protein kinase II (CaMKII), a pivotal enzyme in cellular signaling. The sequence of Autocamtide-2 (KKALRRQETVDAL) closely mimics the phosphorylation site of CaMKII's physiological targets, making it an invaluable tool in studying the kinase's activity, regulation, and role in various biological processes.
CaMKII is a serine/threonine-specific kinase that plays a critical role in synaptic plasticity, learning, memory, and cardiac function. Autocamtide-2 is specifically designed to be efficiently phosphorylated by CaMKII, making it ideal for in vitro assays assessing enzyme kinetics and activity. Its high specificity for CaMKII ensures minimal interference from other kinases, allowing for precise measurements in experimental systems.
In neuroscience research, Autocamtide-2 is extensively used to explore CaMKII's role in synaptic transmission and neuroplasticity. By studying its phosphorylation, researchers gain insights into how CaMKII contributes to long-term potentiation (LTP) and long-term depression (LTD), critical mechanisms underlying memory formation and cognitive function.
In cardiovascular studies, Autocamtide-2 aids in understanding CaMKII's involvement in heart rhythm regulation and pathological conditions such as arrhythmias and heart failure. By serving as a reliable substrate, it enables the investigation of CaMKII inhibitors and their potential therapeutic applications.
Autocamtide-2's robust application extends to high-throughput screening assays for CaMKII-targeting compounds, contributing to drug discovery efforts. Its stability and well-characterized properties make it a cornerstone in kinase research.
Overall, Autocamtide-2 remains an essential peptide for elucidating the complex regulatory mechanisms of CaMKII and advancing therapeutic strategies targeting this critical enzyme in neurobiology and cardiology.

Reference:

Matsumoto, T., Okuwaki, K., Imaizumi, H., Kida, M., Iwai, T., Yamauchi, H., ... & Koizumi, W. Autocamtide 2 chemical.