Caspase 1 (ICE) Substrate 2, chromogenic

Product Name: Caspase 1 (ICE) Substrate 2, chromogenic

Sequence One Letter Code: Ac-YVAD-pNA

Sequence Three Letter Code: Ac-Tyr-Val-Ala-Asp-pNA

Cas No: 149231-66-3

Chemical Formula:C29H36N6O10

Molecular Weight: 628.7

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

SMILES: C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC2=CC=C(C=C2)O)NC(=O)C

IUPAC: (3S)-3-[[(2S)-2-[[(2S)-2-[[(2S)-2-acetamido-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-(4-nitroanilino)-4-oxobutanoic acid

INCHIKEY: YDPNOCSPPGFBPX-XNHCRPTKSA-N

INCHI:

InChI=1S/C29H36N6O10/c1-15(2)25(34-28(42)22(31-17(4)36)13-18-5-11-21(37)12-6-18)29(43)30-16(3)26(40)33-23(14-24(38)39)27(41)32-19-7-9-20(10-8-19)35(44)45/h5-12,15-16,22-23,25,37H,13-14H2,1-4H3,(H,30,43)(H,31,36)(H,32,41)(H,33,40)(H,34,42)(H,38,39)/t16-,22-,23-,25-/m0/s1

Source / Species:

Conjugation: Conjugated

Conjugation Type: Conjugates

Code Nacres: NA.26

Application: Caspase-1 Chromogenic Substrate enables colorimetric detection of caspase-1 activity, a key enzyme in inflammasome signaling, cytokine maturation, and inflammatory proteolysis. Upon enzymatic cleavage, the substrate releases p-nitroaniline, producing an absorbance signal measurable around 405 nm. This non-fluorescent assay format provides convenient quantitative monitoring of caspase-1 activation in biochemical studies. Caspase-1 Chromogenic Substrate is widely used in inflammation research, pyroptosis studies, inflammasome pathway analysis, cytokine processing research, and screening of caspase-1 modulators or inhibitors targeting inflammatory disease mechanisms.