Product Name: Fomc-Gly-Gly-Phe-Gly-OH
Sequence: Fomc-Gly-Gly-Phe-Gly
Purity: 99%
Form: White to off-white Solid
Storage : Sealed storage, away from moisture
CAS.NO.: 1817857-75-2
CHEMICAl FORMULA: C30H30N4O7
Molar Mass: 558.58
SMILES: C1=CC=C(C=C1)C[C@@H](C(=O)NCC(=O)O)NC(=O)CNC(=O)CNC(=O)OCC2C3=CC=CC=C3C4=CC=CC=C24
IUPACNAME: 2-[[(2S)-2-[[2-[[2-(9H-fluoren-9-ylmethoxycarbonylamino)acetyl]amino]acetyl]amino]-3-phenylpropanoyl]amino]acetic acid
INCHIKEY: LCOCGQVZUSLQJD-VWLOTQADSA-N
INCHI: InChI=1S/C30H30N4O7/c35-26(31-16-27(36)34-25(29(39)32-17-28(37)38)14-19-8-2-1-3-9-19)15-33-30(40)41-18-24-22-12-6-4-10-20(22)21-11-5-7-13-23(21)24/h1-13,24-25H,14-18H2,(H,31,35)(H,32,39)(H,33,40)(H,34,36)(H,37,38)/t25-/m0/s1
Application: Boc-Gly-Gly-Phe-Gly-OH is an N- and C-protected tetrapeptide linker designed for antibody-drug conjugate (ADC) research and peptide-based drug delivery applications. This Gly-Gly-Phe-Gly sequence functions as a protease-cleavable linker, enabling enzymatic release of conjugated payloads under specific biological conditions. Its protected structure supports controlled synthesis, conjugation, and incorporation into complex molecular architectures. Boc-Gly-Gly-Phe-Gly-OH is valuable for studying linker stability, payload release mechanisms, and ADC design strategies. It is widely used in medicinal chemistry, chemical biology, peptide synthesis, and targeted therapy research focused on cleavable linker systems and antibody-mediated drug delivery.
Current Research: Fmoc-Gly-Gly-Phe-Gly-OH, is a protected tetrapeptide intermediate used in antibody–drug conjugate, peptide–drug conjugate, and bioconjugation research. The sequence Gly-Gly-Phe-Gly provides a compact peptide linker motif, while the Fmoc protecting group enables controlled synthetic manipulation during stepwise linker construction. This compound is especially relevant for the synthesis of ADC dual-drug-linker systems, where precise linker architecture is required to connect targeting units, release motifs, branching structures, and drug payloads in a reproducible manner. A major application of Fmoc-Gly-Gly-Phe-Gly-OH is ADC linker synthesis. Antibody–drug conjugates require linkers that balance plasma stability, intracellular release, payload compatibility, hydrophilicity, and conjugation efficiency. Peptide-based linkers are widely studied because they can be cleaved by intracellular or tumor-associated proteases, allowing payload release after antibody-mediated internalization. The Gly-Gly-Phe-Gly motif provides a defined peptide segment that can be incorporated into more complex cleavable linker systems for controlled drug release studies. The Fmoc protecting group is central to the synthetic utility of this intermediate. Fmoc chemistry is widely used in solid-phase peptide synthesis and solution-phase peptide fragment assembly because it protects the amino terminus while allowing selective deprotection under mild basic conditions. In Fmoc-Gly-Gly-Phe-Gly-OH, the protected N-terminus and free C-terminal carboxylic acid allow the compound to be coupled to amine-containing spacers, payload modules, branching units, self-immolative groups, or additional peptide segments. This makes it a flexible intermediate for modular ADC linker construction. Fmoc-Gly-Gly-Phe-Gly-OH is particularly valuable in dual-drug-linker research. Dual-drug ADCs are designed to deliver two payloads from one targeting platform or to incorporate two different cytotoxic mechanisms in one conjugate. This strategy introduces additional structural challenges, including linker branching, payload ratio control, differential release, solubility management, and stability optimization. A defined tetrapeptide intermediate such as Fmoc-Gly-Gly-Phe-Gly-OH can function as part of the linker assembly that supports ordered construction of dual-payload architectures. The compound is also associated with GGFGE-related linker assembly strategies. The GGFGE motif or intermediate-derived linker unit can contribute to the formation of an important ADC dual-drug-link assembly module. In this context, Fmoc-Gly-Gly-Phe-Gly-OH may serve as a precursor or fragment that is extended, coupled, or modified to generate a more complex linker sequence. Such assembly units are valuable because ADC synthesis requires high structural consistency, controlled stoichiometry, and predictable behavior during conjugation and purification. Protease-cleavable linker research is another important use. Peptide linkers are often designed to respond to lysosomal proteases after ADC internalization. Cleavage efficiency depends on amino acid sequence, steric accessibility, neighboring spacer chemistry, payload bulk, antibody conjugation site, and linker conformation. Fmoc-Gly-Gly-Phe-Gly-OH can support synthesis of model substrates or linker-payload constructs used to evaluate protease sensitivity, cleavage kinetics, and payload release profiles. LC-MS, HPLC, fluorescence assays, and cell lysate cleavage studies are commonly used to analyze these systems. The Gly-Gly-Phe-Gly sequence has useful structural features. Glycine residues provide flexibility and reduce steric congestion, while phenylalanine introduces aromatic hydrophobic character that may influence enzyme recognition, local conformation, and self-association behavior. This combination makes the motif useful for exploring how peptide sequence affects linker cleavage and physicochemical performance. Researchers may compare Gly-Gly-Phe-Gly-based linkers with Val-Cit, Phe-Lys, Gly-Phe-Leu-Gly, Ala-Ala-Asn, or other protease-responsive motifs to optimize ADC release behavior. Fmoc-Gly-Gly-Phe-Gly-OH also supports peptide–drug conjugate and targeted delivery research beyond classical antibodies. Peptide linkers can be incorporated into small protein conjugates, antibody fragments, nanobodies, peptide-targeted payloads, polymer–drug conjugates, nanoparticle systems, and imaging probes. In these systems, a cleavable peptide linker can help release a payload after cellular uptake or exposure to enzyme-rich compartments. In synthetic chemistry workflows, this intermediate supports route optimization and linker library development. Researchers may use it to build analog panels with different terminal spacers, hydrophilic modifiers, self-immolative groups, branching handles, or payload attachment chemistries. Analytical confirmation typically includes HPLC purity assessment, LC-MS identity confirmation, NMR characterization where needed, and monitoring of coupling efficiency during multistep synthesis. Solubility and aggregation are key considerations in ADC linker design. Peptide fragments containing aromatic residues and hydrophobic payloads can increase aggregation risk. Therefore, Fmoc-Gly-Gly-Phe-Gly-OH-derived linkers may be combined with hydrophilic spacers such as PEG, polysarcosine, charged amino acids, or other solubilizing units to improve conjugate handling. This is especially important in dual-drug-linker systems, where payload hydrophobicity can be amplified. Recommended research controls include non-cleavable linker analogs, scrambled peptide linker analogs, single-drug linker comparators, enzyme-free controls, heat-inactivated protease controls, lysosomal extract cleavage assays, serum stability studies, and LC-MS confirmation of released fragments. For dual-drug systems, researchers should also evaluate payload ratio, release sequence, linker stability, aggregation, conjugation efficiency, and biological activity after assembly. Overall, Fmoc-Gly-Gly-Phe-Gly-OH, Compound D5, is a valuable protected peptide intermediate for ADC and bioconjugation research. It supports dual-drug-linker synthesis, GGFGE linker assembly, protease-cleavable linker development, peptide–drug conjugate construction, controlled payload release studies, linker structure–activity optimization, and modular synthesis of next-generation targeted delivery systems.
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