Phospho-Glycogen Synthase Peptide-2 (substrate)

Product Name:Phospho-Glycogen Synthase Peptide-2 (substrate)

CAS No:851366-97-7

Purity:95%

Molar Mass:3029

Chemical Formula:C123H191N40O48P

Storage:Store at -20 degrees Celsius

Sequence:YRRAAVPPSPSLSRHSSPHQXEDEEE

Application:

Phospho-Glycogen Synthase Peptide-2 (substrate) is a synthetic peptide that serves as a substrate for glycogen synthase kinase-3 (GSK-3), an enzyme that plays a crucial role in various cellular processes, including glycogen metabolism, cell signaling, and gene expression. This peptide is derived from the sequence of glycogen synthase, the enzyme responsible for glycogen synthesis. The inclusion of a phosphate group in the peptide mimics the phosphorylated state of glycogen synthase, making it an ideal substrate for studying GSK-3 activity in vitro. Researchers use Phospho-Glycogen Synthase Peptide-2 to investigate GSK-3's role in metabolic regulation, cell signaling pathways, and its implications in diseases such as diabetes, neurodegenerative disorders, and cancer.

Current Research:

Phospho-Glycogen Synthase Peptide-2: A Substrate for Kinase Activity Studies Phospho-Glycogen Synthase Peptide-2 (PGS-2) is a synthetic peptide substrate derived from the phosphorylation site region of glycogen synthase, an enzyme crucial for glycogen biosynthesis. This peptide mimics a specific phosphorylation site and is commonly used in biochemical assays to study the activity of kinases involved in glycogen metabolism, such as glycogen synthase kinase-3 (GSK-3) and protein kinase A (PKA). Structure and Features Sequence: The peptide sequence typically contains serine or threonine residues in a consensus motif recognized by glycogen-metabolizing kinases. Phosphorylation Site: It features a phosphorylated residue that serves as a substrate for kinases, enabling the study of kinase activity under controlled conditions. Applications Kinase Activity Assays: Used as a substrate in enzymatic assays to measure the activity of GSK-3, PKA, and other kinases. Assays typically monitor the incorporation of phosphate groups using radiolabeled ATP or phosphorylation-specific antibodies. Drug Discovery: Employed in high-throughput screening to identify kinase inhibitors for therapeutic targeting in diseases like diabetes, cancer, and neurodegenerative disorders. Biochemical Studies: Used to investigate the regulation of glycogen synthase by phosphorylation and the role of kinases in metabolic pathways. Signal Transduction Research: Aids in elucidating pathways involving glycogen metabolism and energy homeostasis. Handling and Storage Lyophilized Form: Supplied as a stable lyophilized powder for long-term storage. Storage Conditions: Store at -20 degrees Celsius in a desiccated environment to preserve activity. Reconstitution: Reconstitute in sterile water or assay buffer appropriate for the experimental conditions. Conclusion Phospho-Glycogen Synthase Peptide-2 is a valuable tool in biochemical research for studying kinase activity, signal transduction, and glycogen metabolism. Its use in assays and drug discovery contributes to advancements in understanding and treating metabolic and kinase-related diseases.

Reference:

Dieni, C. A., Bouffard, M. C., & Storey, K. B. (2012). Glycogen synthase kinase-3: cryoprotection and glycogen metabolism in the freeze-tolerant wood frog. Journal of Experimental Biology, 215(3), 543-551.