Product Name:RR-src
CAS No:81156-93-6
Purity:95%
Molar Mass:1519.7
Chemical Formula:C64H106N22O21
Storage:Store at -20 degrees Celsius
Sequence:RRLIEDAEYAARG
Application:
RR-src is a synthetic peptide substrate derived from a sequence within the Src kinase, a non-receptor tyrosine kinase involved in the regulation of various cellular processes such as growth, differentiation, and survival. The sequence typically includes multiple arginine residues (RR) to enhance the peptide's affinity for the enzyme. RR-src is used primarily in biochemical assays to study the activity of Src kinase and other related kinases. By monitoring the phosphorylation of this peptide, researchers can gain insights into kinase signaling pathways, enzyme kinetics, and the effects of potential inhibitors or activators. This makes RR-src a valuable tool in cancer research, where aberrant Src kinase activity is often implicated in tumor progression and metastasis, as well as in the development of targeted therapies.
Current Research:
RR-src (CAS: 81156-93-6) is a synthetic peptide substrate specifically designed for assessing the activity of tyrosine-specific protein kinases, notably the Src family kinases. Its amino acid sequence, Arg-Arg-Leu-Ile-Glu-Asp-Ala-Glu-Tyr-Ala-Ala-Arg-Gly (RRLIEDAEYAARG), includes a central tyrosine residue that serves as the primary site for phosphorylation by these kinases. In biochemical assays, RR-src is utilized to monitor kinase activity by measuring the incorporation of phosphate groups into the peptide. This is typically achieved through radiolabeled ATP or phospho-specific antibodies, allowing for quantitative analysis of kinase function. Such assays are instrumental in elucidating signaling pathways and in the development of kinase inhibitors for therapeutic purposes. The peptide's efficacy as a substrate for Src kinases has been validated in various studies, where it facilitates the investigation of kinase-substrate interactions and the kinetic properties of enzyme activity. Its application extends to high-throughput screening platforms aimed at identifying potential modulators of kinase activity. For optimal stability and preservation of activity, RR-src should be stored at -20 degrees Celsius in a desiccated environment. Proper handling is essential to maintain its integrity for experimental use. In summary, RR-src is a valuable tool in molecular biology and biochemistry for probing the functions of tyrosine-specific protein kinases, contributing to a deeper understanding of cellular signaling mechanisms and aiding in drug discovery efforts targeting kinase-related disorders.
Reference:
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