Syk Kinase Peptide Substrate

Product Name:Syk Kinase Peptide Substrate

CAS No:865778-47-8

Purity:95%

Molar Mass:1463.6

Chemical Formula:C66H94N16O22

Storage:Store at -20 degrees Celsius

Sequence:KEDPDYEWPSAK-NH2

Application:Syk Kinase Peptide Substrate is a synthetic peptide used in biochemical assays to study the activity of Syk kinase, a non-receptor tyrosine kinase involved in signaling pathways related to immune cell activation, inflammation, and allergic responses. Syk kinase plays a critical role in the activation of B cells and other immune cells by phosphorylating tyrosine residues on specific substrates. This peptide substrate is often used in research focused on understanding Syk kinase's role in immune signaling, as well as in the development of inhibitors targeting autoimmune diseases, inflammatory conditions, and certain cancers.

Current Research:

Spleen tyrosine kinase (Syk) is a non-receptor tyrosine kinase integral to various cellular signaling pathways, particularly in hematopoietic cells. It plays a pivotal role in immune cell activation, differentiation, and proliferation. Identifying specific peptide substrates for Syk is essential for understanding its enzymatic activity and regulatory mechanisms.
Identified Peptide Substrates
Research has identified several peptide substrates that Syk phosphorylates:
Immunoreceptor Tyrosine-based Activation Motif (ITAM) Peptides: Syk is activated by binding to phosphorylated ITAM sequences present in immune receptors. Synthetic peptides mimicking these phosphorylated ITAMs can serve as substrates for Syk, facilitating studies on its activation and function.
Biotin-Gastrin Precursor (Tyr87) Peptide: Commercial kinase assay kits utilize biotinylated peptides, such as the Biotin-Gastrin Precursor (Tyr87), as substrates to measure Syk activity. These peptides, upon phosphorylation by Syk, can be detected using phospho-tyrosine-specific antibodies, enabling quantitative analysis of kinase activity.
Applications in Research
Utilizing these peptide substrates allows researchers to:
Assess Syk Activity: By measuring the phosphorylation levels of these substrates, scientists can evaluate Syk's kinase activity under various experimental conditions.
Screen Inhibitors: Peptide substrates are employed in high-throughput screening assays to identify potential Syk inhibitors, which could serve as therapeutic agents for diseases involving aberrant Syk activity.
Elucidate Signaling Pathways: Studying the phosphorylation of specific substrates helps delineate the signaling cascades mediated by Syk, enhancing our understanding of immune cell regulation.
Conclusion
Identifying and utilizing specific peptide substrates for Syk kinase are fundamental for probing its biological functions and regulatory mechanisms. These substrates not only facilitate the assessment of Syk activity but also aid in the development of targeted therapies for conditions where Syk plays a critical role.

Reference:

Lipchik, A. M., Killins, R. L., Geahlen, R. L., & Parker, L. L. (2012). A peptide-based biosensor assay to detect intracellular Syk kinase activation and inhibition. Biochemistry, 51(38), 7515-7524.