Akt/SKG Substrate Peptide

Product Name:Akt/SKG Substrate Peptide

CAS No:276680-69-4

Purity:95%

Molar Mass:817.9

Chemical Formula:C36H59N13O9

Storage:Store at -20 degrees Celsius

Sequence:RPRAATF

Target:Akt

Application:

Akt/SKG Substrate Peptide is a synthetic peptide designed to be a specific substrate for the serine/threonine kinase Akt (also known as Protein Kinase B, or PKB). Akt is a key player in various cellular processes, including glucose metabolism, cell proliferation, apoptosis, and survival. The Akt/SKG Substrate Peptide contains a sequence that mimics the natural phosphorylation site targeted by Akt, allowing researchers to study the enzyme's kinase activity in vitro. This peptide is used in assays to measure Akt activity, which is crucial for understanding the role of Akt in signaling pathways related to cancer, diabetes, and other diseases. It helps in evaluating the effects of potential therapeutic agents that modulate Akt activity.

Current Research:

Akt/SKG Substrate Peptide: A Tool for Kinase Activity Studies Akt/SKG Substrate Peptide is a synthetic peptide designed as a specific substrate for the serine/threonine kinase Akt (also known as protein kinase B). It mimics the consensus phosphorylation motif recognized by Akt, making it an essential tool for studying Akt-mediated phosphorylation in vitro. Structure and Features Sequence: The peptide contains the Akt recognition sequence, typically RPRAATF or similar, with a serine or threonine residue as the phosphorylation site. Functionality: This sequence is specifically phosphorylated by Akt, enabling the investigation of Akt activity and its role in various signaling pathways. Applications Kinase Activity Assays: The Akt/SKG Substrate Peptide is widely used in biochemical assays to measure Akt kinase activity. Phosphorylation can be detected using radiolabeled ATP or phosphorylation-specific antibodies. Signal Transduction Research: Akt is a central node in pathways regulating cell survival, growth, and metabolism. This peptide is crucial for studying these pathways in detail. Used to investigate the downstream effects of Akt activation in physiological and pathological contexts. Drug Discovery: The peptide serves as a substrate in high-throughput screening assays to identify inhibitors of Akt. Such inhibitors are of therapeutic interest in cancer, diabetes, and other diseases. Phosphorylation Studies: Enables the characterization of phosphorylation kinetics and the influence of cofactors or inhibitors on Akt activity. Handling and Storage Lyophilized Form: Supplied as a stable lyophilized powder. Storage: Store at -20 degrees Celsius in a desiccated environment to maintain activity. Reconstitution: Reconstitute in a kinase assay buffer or sterile water, depending on the experimental setup. Conclusion The Akt/SKG Substrate Peptide is an indispensable tool in molecular biology and biochemistry for investigating Akt kinase activity and its role in cellular signaling. Its specificity and versatility make it a key reagent in studies of signal transduction, metabolic regulation, and drug development.

Reference:

Henschke, S., Nolte, H., Magoley, J., Kleele, T., Brandt, C., Hausen, A. C., … & Brüning, J. C. (2024). Food perception promotes phosphorylation of MFFS131 and mitochondrial fragmentation in liver. Science, 384(6694), 438-446.