Caspase 1 (ICE) Substrate 3f, fluorogenic

Product Name: Caspase 1 (ICE) Substrate 3f, fluorogenic

Sequence One Letter Code: Ac-WEHD-AFC

Sequence Three Letter Code: Ac-Trp-Glu-His-Asp-AFC

Cas No: 210344-99-3

Chemical Formula:C38H37F3N8O11

Molecular Weight: 838.8

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

SMILES: CC(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC3=CN=CN3)C(=O)N[C@@H](CC(=O)O)C(=O)NC4=CC5=C(C=C4)C(=CC(=O)O5)C(F)(F)F

IUPAC: (4S)-4-[[(2S)-2-acetamido-3-(1H-indol-3-yl)propanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid

INCHIKEY: NEGOLAGPKZGIKJ-DZUOILHNSA-N

INCHI:

InChI=1S/C38H37F3N8O11/c1-18(50)45-27(10-19-15-43-25-5-3-2-4-22(19)25)36(58)47-26(8-9-31(51)52)34(56)48-28(11-21-16-42-17-44-21)37(59)49-29(14-32(53)54)35(57)46-20-6-7-23-24(38(39,40)41)13-33(55)60-30(23)12-20/h2-7,12-13,15-17,26-29,43H,8-11,14H2,1H3,(H,42,44)(H,45,50)(H,46,57)(H,47,58)(H,48,56)(H,49,59)(H,51,52)(H,53,54)/t26-,27-,28-,29-/m0/s1

Source / Species:

Conjugation: Conjugated

Conjugation Type: Fluorescent dyes

Code Nacres: NA.26

Application: Caspase-1 Fluorogenic Substrate is designed for sensitive measurement of caspase-1, also known as interleukin-1β-converting enzyme or ICE. Caspase-1 is a key mediator of inflammasome activation and plays a central role in processing inflammatory cytokines such as IL-1β and IL-18. Upon enzymatic cleavage, the AFC fluorophore is released, producing strong fluorescence detectable at excitation 370–390 nm and emission 500–510 nm. This substrate is widely used in inflammation research, pyroptosis studies, inflammasome signaling analysis, caspase-1 inhibitor screening, and biochemical assays measuring inflammatory protease activity.