Fluorescein-Trp25-Exendin-4 (FLEX)

Product Name: Fluorescein-Trp25-Exendin-4 (FLEX)

Sequence One Letter Code: HGEGTFTSDLSKQMEEEAVRLFIE-(Trp-S-FAM)-LKNGGPSSGAPPPS-NH2

Sequence Three Letter Code: H-His-Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-(Trp-S-FAM)-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2

Molecular Weight: 4606.4

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

Source / Species: Gila

Conjugation: Conjugated

Conjugation Type: Fluorescent dyes

Code Nacres: NA.26

Application: Fluorescein-Trp25-Exendin-4, also known as FLEX, is a fluorescently labeled analog of Exendin-4 with fluorescein conjugated at the tryptophan-25 residue. FLEX retains full biological activity and shows binding affinity comparable to native GLP-1 and Exendin-4 at the human GLP-1 receptor. It stimulates cAMP accumulation and displays specific, saturable receptor binding, supporting quantitative analysis of ligand–receptor interactions. FLEX is widely used in fluorescence anisotropy, receptor binding assays, ligand competition studies, and functional signaling assays. This peptide is valuable for GLP-1 receptor pharmacology, incretin signaling, and metabolic disease research.