EMP17, FITC-LC labeled

Product Name: EMP17, FITC-LC labeled

Sequence One Letter Code: FITC-LC-TYSCHFGPLTWVCKPQGG

Sequence Three Letter Code: FITC-LC-Thr-Tyr-Ser-Cys-His-Phe-Gly-Pro-Leu-Thr-Trp-Val-Cys-Lys-Pro-Gln-Gly-Gly-OH

Chemical Formula:C117H149N25O30S3

Molecular Weight: 2483.9

Purity: 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

Research Area: Peptide Series

Conjugation: Conjugated

Conjugation Type: Fluorescent dyes

Code Nacres: NA.26

Application: EMP17, FITC-LC labeled, is a fluorescent erythropoietin-mimetic peptide designed for receptor binding and signaling analysis. By engaging the extracellular domain of the erythropoietin receptor and promoting receptor dimerization, it models key aspects of EPO-mediated signal transduction. FITC conjugation via a long-chain linker enables fluorescence detection at Ex/Em 494/520 nm. This peptide is applied in studies of receptor–ligand dynamics, erythropoietic signaling, and fluorescence-based binding assays.

Current Research: EMP17, FITC-LC labeled is a fluorescently tagged erythropoietin (EPO)-mimetic peptide engineered for analysis of erythropoietin receptor (EPOR) binding and signaling. EMP17 is designed to engage the extracellular domain of EPOR and promote receptor dimerization, thereby modeling critical aspects of native EPO-induced signal transduction. Conjugation with fluorescein isothiocyanate (FITC) via a long-chain (LC) spacer enables sensitive fluorescence detection while minimizing steric interference with receptor interaction. The labeled peptide exhibits fluorescence at approximately Ex 494 nm / Em 520 nm, compatible with standard FITC filter settings. Biological Context The erythropoietin receptor (EPOR) is a class I cytokine receptor that mediates erythropoiesis. Ligand binding induces receptor dimerization and activation of associated Janus kinase 2 (JAK2), leading to: STAT5 phosphorylation and nuclear translocation Activation of MAPK/ERK pathways PI3K/Akt signaling Promotion of erythroid progenitor survival and differentiation EMP17 functions as a peptide-based surrogate ligand, reproducing receptor engagement and dimerization events necessary for downstream signaling activation. Functional Advantages of FITC-LC Labeling The FITC fluorophore provides: Direct visualization without secondary labeling Compatibility with flow cytometry and fluorescence microscopy Quantitative measurement in plate-based fluorescence assays The long-chain linker improves accessibility of the peptide to EPOR while reducing potential steric hindrance from the fluorophore. Research Applications 1. Receptor Binding and Affinity Analysis EMP17–FITC is used in competitive binding assays to quantify EPOR affinity and evaluate displacement by native EPO or receptor antagonists. 2. Receptor Dimerization and Signaling Studies The peptide supports investigation of receptor activation dynamics, including JAK2 phosphorylation, STAT signaling, and downstream transcriptional responses. 3. Flow Cytometry-Based Binding Assays Fluorescent labeling allows direct measurement of ligand binding to EPOR-expressing cells and quantification of receptor density or occupancy. 4. Imaging and Internalization Studies Fluorescence microscopy can be used to monitor receptor–ligand internalization, trafficking, and spatial distribution within cells. 5. Erythropoietic Signaling Research EMP17 provides a controllable model system for studying erythroid lineage survival pathways and cytokine receptor signaling mechanisms. Experimental Considerations Fluorophore stability requires protection from prolonged light exposure. Validation against native EPO may be necessary when assessing signaling potency. Receptor expression levels should be confirmed to ensure accurate interpretation of binding assays.