Glandular Kallikrein Substrate, fluorescent H-D-Val-Leu-Arg-AFC

Product Name: Glandular Kallikrein Substrate, fluorescent H-D-Val-Leu-Arg-AFC

Sequence One Letter Code: vLR-AFC

Sequence Three Letter Code: H-D-Val-Leu-Arg-AFC

Chemical Formula:C27H38F3N7O5

Molecular Weight: 597.7

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

Source / Species: Synthetic construct

Conjugation: Conjugated

Conjugation Type: Fluorescent dyes

Code Nacres: NA.26

Application: H-D-Val-Leu-Arg-AFC is a fluorogenic peptide substrate designed for measuring glandular kallikrein activity. Upon enzymatic cleavage, the AFC fluorophore is released, producing fluorescence detectable at Abs/Em 380/500 nm. Kallikreins are serine proteases involved in inflammation, blood pressure regulation, tissue remodeling, renal physiology, and cancer-associated pathways. This substrate enables sensitive quantitative monitoring of kallikrein-related peptidase activity in biochemical assays. H-D-Val-Leu-Arg-AFC is widely used in enzymatic kinetics, inhibitor screening, protease profiling, cardiovascular research, renal studies, and investigations of kallikrein-mediated biological regulation.