Scrambled 10Panx

Product Name:Scrambled 10Panx

CAS No:1315378-72-3

Purity:95%

Molar Mass:1242.37

Chemical Formula:C58H79N15O16

Storage:Store at -20 degrees Celsius

Sequence:FSVYWAQADR

Application:

Scrambled 10Panx refers to a variant of the 10-amino acid peptide derived from the pannexin-1 (Panx1) channel protein. The "scrambled" version means that the amino acid sequence has been rearranged compared to the native sequence. Pannexin-1 is involved in cell signaling through the formation of hemichannels that mediate ATP release and intercellular communication. The scrambled peptide is used as a control in research to study the specific interactions and functions of the native Panx1 peptide. It helps differentiate between the effects of the specific Panx1 peptide and non-specific effects due to sequence scrambling.

Current Research:

Scrambled 10Panx (CAS: 1315378-72-3) is a control peptide derived from the sequence of 10Panx, a pannexin-1 (Panx1) mimetic inhibitory peptide. The sequence of Scrambled 10Panx is FSVYWAQADR, a randomized arrangement of the amino acids in 10Panx. This scrambled variant is utilized in research to discern the specific effects of 10Panx by serving as a negative control, ensuring that observed biological activities are due to the specific sequence of 10Panx rather than nonspecific peptide interactions.
Pannexin-1 is a membrane channel protein implicated in various physiological and pathological processes, including ATP release and intercellular communication. Inhibitory peptides like 10Panx are designed to block Panx1 channels, thereby modulating these processes. The use of Scrambled 10Panx as a control is crucial in experiments to validate the specificity of 10Panx's inhibitory actions on Panx1 channels.
Studies have demonstrated that while 10Panx effectively inhibits dye uptake and ATP release in macrophages, Scrambled 10Panx does not produce these effects, underscoring the sequence-specific activity of 10Panx. This highlights the importance of using appropriate control peptides in experimental setups to accurately attribute biological effects to targeted interventions.
In summary, Scrambled 10Panx serves as an essential tool in the study of Panx1 channel functions, providing a baseline to distinguish specific inhibitory effects of 10Panx from nonspecific peptide interactions. Its application enhances the rigor and validity of research involving pannexin-1 modulation.

Reference:

Thompson, R. J., Jackson, M. F., Olah, M. E., Rungta, R. L., Hines, D. J., Beazely, M. A., ... & MacVicar, B. A. (2008). Activation of pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science, 322(5907), 1555-1559.