Product Name:ATTO488-ProTx-II
Purity:95%
Molar Mass:3826.66
Chemical Formula:C168H250N46O41S8
Storage:Store at -20 degrees Celsius
Sequence:Tyr-Cys2-Gln-Lys-Trp-Met-Trp-Thr-Cys9-Asp-Ser-Glu-Arg-Lys-Cys15-Cys16-Glu-Gly-Met-Val-Cys21-Arg-Leu-Trp-Cys25-Lys-Lys-Lys-Leu-Trp-OH
Target:NaV1.7 channels
Application:
ATTO488-ProTx-II is a fluorescently labeled version of the peptide toxin ProTx-II, which is derived from the venom of the tarantula Thrixopelma pruriens. ProTx-II is known for its high selectivity in inhibiting the voltage-gated sodium channel Nav1.7, a key player in pain perception. By conjugating ProTx-II with the fluorescent dye ATTO488, researchers can visualize and study its interaction with sodium channels in live cells or tissues. This modified peptide is widely used in fluorescence microscopy and binding assays, helping to explore the mechanisms of sodium channel modulation and to aid in the development of new pain therapeutics targeting Nav1.7. ATTO488’s bright fluorescence provides high sensitivity, making it ideal for real-time imaging studies in neurobiology and pharmacology.
Current Research:
ATTO488-ProTx-II is a fluorescently labeled derivative of ProTx-II, a peptide toxin originally isolated from the venom of the Peruvian green velvet tarantula (Thrixopelma pruriens). ProTx-II is known for its high selectivity in inhibiting the voltage-gated sodium channel Naᵥ1.7, a key player in pain perception. By conjugating ProTx-II with the fluorescent dye ATTO488, researchers can visualize and study its interaction with sodium channels in live cells or tissues.
Structural and Functional Characteristics
ProTx-II is a 30-amino-acid peptide that adopts an inhibitor cystine knot (ICK) motif, contributing to its stability and affinity for sodium channels. The addition of the ATTO488 fluorophore does not significantly alter the peptide's binding affinity or inhibitory potency. ATTO488-ProTx-II retains high specificity for Naᵥ1.7 channels, with an IC₅₀ value of approximately 0.3 nM, and exhibits at least 100-fold selectivity over other sodium channel subtypes.
Applications in Research
The fluorescent properties of ATTO488-ProTx-II make it an invaluable tool in neurophysiological and pharmacological research:
Visualization of Channel Distribution: ATTO488-ProTx-II facilitates the mapping of Naᵥ1.7 channel distribution in tissues and cells, enhancing the understanding of channel localization and density.
Live-Cell Imaging: The ATTO488 label allows for real-time observation of toxin-channel interactions in living cells, providing insights into the dynamics of channel function and regulation.
Drug Development: ATTO488-ProTx-II serves as a probe in high-throughput screening assays aimed at identifying novel modulators of Naᵥ1.7 channels, which are potential therapeutic targets for pain management.
Handling and Storage
ATTO488-ProTx-II is typically supplied as a lyophilized powder. For optimal stability, it should be stored at -20°C, protected from light and moisture. Upon reconstitution, aliquots should be prepared to avoid repeated freeze-thaw cycles, which can degrade the peptide.
Conclusion
ATTO488-ProTx-II combines the potent inhibitory properties of ProTx-II with the fluorescent capabilities of the ATTO488 dye, offering researchers a powerful tool to study Naᵥ1.7 sodium channels. Its application enhances the visualization and understanding of sodium channel physiology, contributing to advancements in neurobiology and the development of pain therapeutics.
Reference:
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