BIM, Biotinylated

Product Name:BIM, Biotinylated

Purity:95%

Molar Mass:3637.14

Chemical Formula:C161H247N49O44S2

Storage:Store at -20 degrees Celsius

Sequence:AADMRPEIWIAQELRRIGDEFNAYYARR

Application:Biotinylated BIM is a peptide variant of the BIM (Bcl-2-interacting mediator) protein, with biotin conjugated to it. BIM is a pro-apoptotic member of the Bcl-2 family that plays a crucial role in the regulation of apoptosis (programmed cell death). The biotinylation allows for easy detection and isolation of BIM-bound complexes through streptavidin-based assays. This biotinylated form is used in research to study the interactions between BIM and its target proteins, as well as to investigate the mechanisms of apoptosis and the role of BIM in cancer and other diseases where apoptosis regulation is crucial.

Current Research:

Biotinylated BIM is a synthetic peptide corresponding to the Bcl-2-interacting mediator of cell death (Bim) protein, modified with a biotin moiety. This peptide is derived from residues 81-106 of the Bim sequence, representing the BH3 domain, a key region responsible for interactions with anti-apoptotic proteins like Bcl-2 and Bcl-xL. The biotinylation enables the peptide's precise detection and immobilization in biochemical assays, significantly enhancing its utility in apoptosis research.

Biological Significance

Bim is a pro-apoptotic member of the Bcl-2 protein family, which regulates programmed cell death by controlling mitochondrial outer membrane permeabilization (MOMP). It activates apoptotic pathways by binding to and neutralizing anti-apoptotic proteins, allowing pro-apoptotic effectors such as Bax and Bak to initiate cytochrome c release. Dysregulation of Bim-mediated pathways is implicated in various diseases, including cancer, neurodegeneration, and autoimmune disorders.

Current Research Areas

Protein-Protein Interaction Studies: Biotinylated BIM is widely used in binding assays to study the interaction of the Bim BH3 domain with Bcl-2 family proteins. These studies provide insights into the molecular mechanisms of apoptotic regulation.

Apoptosis Mechanism Elucidation: Researchers employ the peptide to investigate how Bim disrupts anti-apoptotic complexes, initiating the apoptotic cascade. These findings are crucial for understanding cell death in both normal physiology and disease.

Drug Discovery: The peptide is instrumental in high-throughput screening for small molecules targeting Bim interactions. Such efforts aim to develop novel therapies for conditions like cancer, where apoptosis resistance is a hallmark.

Applications and Potential

Biotinylated BIM supports the development of apoptosis-targeting therapeutics by enabling the identification and characterization of potential inhibitors or enhancers of Bim interactions. Its role in deciphering apoptotic mechanisms positions it as a vital tool in both basic and translational research.

Conclusion

Biotinylated BIM continues to drive advancements in apoptosis research, contributing to the understanding of cell death pathways and the development of targeted therapies for apoptosis-related diseases.

Reference:

Edwards, A. L., Wachter, F., Lammert, M., Huhn, A. J., Luccarelli, J., Bird, G. H., & Walensky, L. D. (2015). Cellular uptake and ultrastructural localization underlie the pro-apoptotic activity of a hydrocarbon-stapled BIM BH3 peptide. ACS chemical biology, 10(9), 2149-2157.