CALP3

Product Name:CALP3

CAS No:261969-05-5

Purity:95%

Molar Mass:881.08

Chemical Formula:C44H68N10O9

Storage:Store at -20 degrees Celsius

Sequence:VKFGVGFK

Target:calmodulin

Application:

CALP3 is a synthetic peptide designed as an activator or modulator of calpain, a family of calcium-dependent cysteine proteases that play crucial roles in various cellular processes, including cytoskeletal remodeling, signal transduction, and apoptosis. Calpain activity is tightly regulated by calcium levels, and dysregulation of this activity is associated with several pathological conditions, such as neurodegenerative diseases, muscle disorders, and ischemic injury. CALP3 is used in research to study the specific effects of calpain activation on cellular functions, providing insights into the role of calpain in health and disease and potential therapeutic strategies for conditions involving abnormal calpain activity.

Current Research:

CALP3 (CAS: 261969-05-5) is a synthetic, cell-permeable peptide that functions as a calmodulin (CaM) agonist. By binding to the EF-hand Ca²⁺-binding sites of CaM, CALP3 can activate CaM-dependent processes even in the absence of Ca²⁺. This unique capability allows CALP3 to modulate various Ca²⁺-dependent cellular functions.

Mechanism of Action

CALP3 binds to the EF-hand motifs of CaM, mimicking the effect of elevated intracellular Ca²⁺ levels. This interaction leads to the activation of CaM-dependent enzymes, such as phosphodiesterases, independent of Ca²⁺ presence. Additionally, CALP3 can inhibit Ca²⁺-mediated cytotoxicity and apoptosis, with an IC₅₀ of approximately 33 μM.

Biological Activity

Phosphodiesterase Activation: CALP3 activates phosphodiesterase enzymes without the need for Ca²⁺, influencing cyclic nucleotide signaling pathways.
Cytoprotection: By inhibiting Ca²⁺-induced cytotoxicity and apoptosis, CALP3 offers protective effects in cellular models subjected to Ca²⁺ overload.
Research Applications

Due to its ability to modulate CaM activity independently of Ca²⁺, CALP3 is a valuable tool in research areas such as:

Calcium Signaling: Investigating the role of CaM in Ca²⁺-independent signaling pathways.
Neuroprotection: Exploring potential therapeutic strategies for conditions involving Ca²⁺-mediated neuronal damage, such as ischemia or neurodegenerative diseases.
Apoptosis Regulation: Studying mechanisms of cell death and survival in diseases characterized by excessive apoptosis, including AIDS and certain neurodegenerative disorders.
Handling and Storage

For optimal stability, CALP3 should be stored desiccated at -20°C. It is soluble in water up to 1 mg/mL, facilitating its use in various experimental protocols.

Conclusion

CALP3 serves as a potent modulator of CaM activity, enabling researchers to dissect Ca²⁺-independent pathways and develop potential therapeutic approaches for diseases associated with Ca²⁺ dysregulation and apoptosis.

Reference:

Charlton, R., Henderson, M., Richards, J., Hudson, J., Straub, V., Bushby, K., & Barresi, R. (2009). Immunohistochemical analysis of calpain 3: advantages and limitations in diagnosing LGMD2A. Neuromuscular Disorders, 19(7), 449-457.