AY-NH2

Product Name:AY-NH2

CAS No:352017-71-1

Purity:95%

Molar Mass:680.8

Chemical Formula:C34H48N8O7

Storage:Store at -20 degrees Celsius

Sequence:AYPGKF

Application:

AY-NH2 is a potent synthetic analog of neuropeptide Y (NPY), specifically modified at the C-terminal end with an amide group (-NH2). This modification enhances the stability and bioactivity of the peptide, making AY-NH2 a valuable tool in research focused on understanding the role of NPY in regulating appetite, stress responses, and cardiovascular function. By interacting with NPY receptors, particularly Y1 and Y5 subtypes, AY-NH2 is used to investigate the mechanisms underlying metabolic disorders, anxiety, and other physiological processes, contributing to the development of potential therapeutic strategies targeting these pathways.

Current Research:

AY-NH₂ (also known as AYPGKF-NH₂) is a synthetic hexapeptide that functions as a selective agonist for Protease-Activated Receptor 4 (PAR4). Its sequence is Ala-Tyr-Pro-Gly-Lys-Phe-NH₂, with a C-terminal amide modification.
Biological Activity:

PAR4 Activation: AY-NH₂ selectively activates PAR4, a G protein-coupled receptor involved in thrombin-mediated cellular effects. It stimulates platelet aggregation in vitro with an EC₅₀ of approximately 15 μM.

Inflammatory Response: In vivo studies have shown that AY-NH₂ increases paw thickness in rat paw edema models, indicating its role in promoting inflammatory responses.

Research Applications:

Platelet Function Studies: AY-NH₂ is utilized to investigate PAR4's role in platelet aggregation and thrombosis.

Inflammation Models: It serves as a tool to study PAR4-mediated inflammatory pathways in various animal models.

Handling and Storage:

Form: Typically supplied as a lyophilized powder.
Storage: Store at -20°C under desiccated conditions to maintain stability.
Reconstitution: Dissolve in sterile water or an appropriate buffer before use.
Safety Note:

AY-NH₂ is intended for research purposes only and is not approved for human or veterinary use. Proper laboratory safety procedures should be followed when handling this compound.

For more detailed information, refer to the product datasheet provided by suppliers such as R&D Systems.

Reference:

Gomides, L. F., Duarte, I. D., Ferreira, R. G., Perez, A. C., Francischi, J. N., & Klein, A. (2012). Proteinase-activated receptor-4 plays a major role in the recruitment of neutrophils induced by trypsin or carrageenan during pleurisy in mice. Pharmacology, 89(5-6), 275-282.