H3K4(Me2) (1-20)

Product Name: H3K4(Me2) (1-20)

Purity: 95%

Molar Mass: 2211.6

Chemical Formula: C93H171N35O27

Storage: Store at -20??

Sequence: ARTKQTARKSTGGKAPRKQL

Application: H3K4(Me2) (1-20) is a synthetic peptide corresponding to the first 20 amino acids of histone H3, dimethylated at the lysine 4 (K4) residue. This specific post-translational modification is associated with actively transcribed genes and plays a pivotal role in the regulation of gene expression. Dimethylation of H3K4 marks both promoter regions and enhancers, facilitating the recruitment of transcriptional machinery. The H3K4(Me2) (1-20) peptide is a valuable tool in epigenetic research, enabling the study of chromatin dynamics, gene activation, and the molecular mechanisms involved in development, differentiation, and disease progression, particularly in the context of transcriptional regulation.

Current Research:

H3K4(Me) (1-20) is a synthetic peptide representing the first 20 amino acids of histone H3, with monomethylation at lysine 4 (K4). This modification is a key epigenetic marker associated with transcriptional regulation, particularly at enhancer and promoter regions of active genes. Role of H3K4 Monomethylation H3K4 monomethylation is catalyzed by histone methyltransferases such as MLL3/MLL4 and SETD1. It is enriched at active gene enhancers, where it facilitates chromatin accessibility and recruitment of transcriptional machinery. This modification is considered a priming mark for subsequent methylation states (di- or trimethylation), which further enhance transcriptional activity. Applications in Research Enzymatic Activity Studies H3K4(Me) (1-20) is widely used as a substrate in assays studying histone methyltransferases (HMTs) and demethylases (HDMs). By providing a specific methylated context, it allows researchers to investigate enzyme specificity and kinetics. Chromatin Biology This peptide is instrumental in exploring chromatin dynamics, helping to elucidate how monomethylation influences nucleosome stability and transcription factor binding. Epigenetic Drug Discovery As a model substrate, H3K4(Me) (1-20) aids in screening inhibitors or activators of HMTs and HDMs, supporting the development of therapeutic agents targeting epigenetic mechanisms. Implications in Disease Aberrant H3K4 methylation is linked to various diseases, including cancer and neurodevelopmental disorders. Altered levels of H3K4me at enhancers can disrupt gene expression programs, contributing to tumorigenesis or developmental defects. Targeting enzymes involved in H3K4 methylation holds potential for therapeutic intervention. Conclusion H3K4(Me) (1-20) is a valuable tool in epigenetic research, enabling detailed studies of histone methylation and its regulatory impact on gene expression. Its applications span fundamental research to drug discovery, advancing our understanding of chromatin-mediated control in health and disease.

Reference: Ginsburg, D. S., Anlembom, T. E., Wang, J., Patel, S. R., Li, B., & Hinnebusch, A. G. (2014). NuA4 links methylation of histone H3 lysines 4 and 36 to acetylation of histones H4 and H3. Journal of Biological Chemistry, 289(47), 32656-32670.