tcY-NH2

Product Name:tcY-NH2

CAS No:327177-34-4

Purity:95%

Molar Mass:739.9

Chemical Formula:C40H49N7O7

Storage:Store at -20 degrees Celsius

Sequence:YPGKF

Application:

tcY-NH2 is a modified analog of neuropeptide Y (NPY), characterized by specific structural alterations that enhance its binding affinity and selectivity for certain NPY receptor subtypes, particularly Y1 and Y2. The addition of an amide group (-NH2) at the C-terminus increases the peptide's stability and bioactivity. tcY-NH2 is widely used in research to investigate the physiological and pathological roles of NPY, including its involvement in appetite regulation, energy balance, and stress responses. This compound is instrumental in studies focused on understanding the underlying mechanisms of metabolic disorders, anxiety, and cardiovascular diseases, aiding in the exploration of novel therapeutic approaches.

Current Research:

tcY-NH₂, also known as (trans-Cinnamoyl)-YPGKF-NH₂, is a synthetic peptide that functions as a selective antagonist of Protease-Activated Receptor 4 (PAR4). Its sequence is trans-Cinnamoyl-Tyr-Pro-Gly-Lys-Phe-NH₂, featuring a trans-cinnamoyl group at the N-terminus and a C-terminal amide modification.
Biological Activity:

PAR4 Antagonism: tcY-NH₂ effectively inhibits PAR4-mediated responses, such as thrombin- and AY-NH₂-induced platelet aggregation, without affecting ADP-mediated aggregation.

Inhibition of ERK Pathway Activation: By blocking PAR4 activation, tcY-NH₂ prevents the activation of the ERK pathway, thereby influencing processes like aortic vascular smooth muscle cell migration and proliferation in rat models.

Research Applications:

Platelet Aggregation Studies: tcY-NH₂ is utilized to investigate the role of PAR4 in platelet function and thrombotic disorders.

Cardiovascular Research: It aids in understanding PAR4's involvement in vascular smooth muscle cell behavior, contributing to studies on atherosclerosis and restenosis.

Inflammation and Immunology: tcY-NH₂ serves as a tool to explore PAR4's role in inflammatory responses and immune cell regulation.

Handling and Storage:

Form: Typically supplied as a lyophilized powder.
Storage: Store at -20°C in a desiccated environment to maintain stability.
Reconstitution: Dissolve in sterile water or an appropriate buffer before use.
Safety Note:

tcY-NH₂ is intended for research purposes only and is not approved for human or veterinary use. Proper laboratory safety procedures should be followed when handling this compound.

For more detailed information, refer to the product datasheet provided by suppliers such as Isca Biochemicals.

Reference:

Mende, K., Reifart, J., Rosentreter, D., Manukyan, D., Mayr, D., Krombach, F., ... & Khandoga, A. (2014). Targeting platelet migration in the postischemic liver by blocking protease-activated receptor 4. Transplantation, 97(2), 154-160.