H3K4(Me) (1-20)

Product Name: H3K4(Me) (1-20)

Purity: 95%

Molar Mass: 2197.6

Chemical Formula: C92H169N35O27

Storage: Store at -20 degrees celsius

Sequence: ARTKQTARKSTGGKAPRKQL

Application: H3K4(Me) (1-20) is a synthetic peptide corresponding to the first 20 amino acids of histone H3, monomethylated at the lysine 4 (K4) residue. This specific post-translational modification is associated with gene activation and is often found at the promoters of actively transcribed genes. Monomethylation of H3K4 plays a critical role in the regulation of chromatin structure and gene expression, serving as a marker for enhancer elements. The H3K4(Me) (1-20) peptide is an essential tool in epigenetic research, aiding in the study of gene regulation, chromatin remodeling, and the mechanisms underlying development and disease, particularly in the context of transcriptional activation and cell differentiation.

Current Research:

H3K4(Me) (1-20) is a synthetic peptide corresponding to the first 20 amino acids of histone H3, with monomethylation at lysine 4 (K4). This post-translational modification is associated with gene activation and plays a crucial role in the regulation of gene expression. Role of H3K4 Monomethylation in Gene Regulation Monomethylation of H3K4 is catalyzed by specific histone methyltransferases, such as MLL3 and MLL4, and is commonly found at enhancer regions of actively transcribed genes. This modification serves as a marker for transcriptional enhancers, facilitating the recruitment of transcription factors and coactivators necessary for gene expression. Applications of H3K4(Me) (1-20) in Research The H3K4(Me) (1-20) peptide is utilized as a substrate in studies investigating the activity of histone methyltransferases and demethylases. By providing a defined sequence with a specific methylation state, researchers can examine how these enzymes interact with methylated histone tails, thereby enhancing our understanding of epigenetic regulation mechanisms. Implications in Disease and Therapeutics Aberrant methylation at H3K4 is implicated in various diseases, including cancer and neurological disorders. Dysregulation of H3K4 methylation can lead to improper gene expression, contributing to disease progression. Consequently, targeting the enzymes that modulate H3K4 methylation is being explored as a therapeutic strategy in oncology and other fields. Conclusion The H3K4(Me) (1-20) peptide serves as a valuable tool in epigenetic research, facilitating the study of histone modifications and their impact on gene expression. Understanding the dynamics of H3K4 monomethylation is essential for developing novel therapeutic approaches for diseases associated with epigenetic dysregulation.

Reference: Liu, Y., Qin, S., Chen, T. Y., Lei, M., Dhar, S. S., Ho, J. C., ... & Min, J. (2019). Structural insights into trans-histone regulation of H3K4 methylation by unique histone H4 binding of MLL3/4. Nature communications, 10(1), 36.