Caspase 9 Substrate 1f, fluorogenic

Product Name: Caspase 9 Substrate 1f, fluorogenic

Sequence One Letter Code: Ac-LEHD-AFC

Sequence Three Letter Code: Ac-Leu-Glu-His-Asp-AFC

Cas No: 210345-03-2

Chemical Formula:C33H38F3N7O11

Molecular Weight: 765.7 Purity: ≥ 95% Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

SMILES: CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=CN=CN1)C(=O)N[C@@H](CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C

IUPAC: (4S)-4-[[(2S)-2-acetamido-4-methylpentanoyl]amino]-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid

INCHIKEY: HULKIXRFKRCRHD-ZJZGAYNASA-N

INCHI:

InChI=1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,45,46)(H,47,48)/t21-,22-,23-,24-/m0/s1

Conjugation: Conjugated

Conjugation Type: Fluorescent dyes

Code Nacres: NA.26

Application: Caspase-9 Fluorogenic Substrate is designed for sensitive detection of caspase-9 activity, a key initiator enzyme in the intrinsic mitochondrial apoptosis pathway. Upon enzymatic cleavage, the AFC fluorophore is released, generating strong fluorescence measurable at excitation 370–390 nm and emission 500–510 nm. Caspase-9 activation triggers downstream executioner caspases, making this substrate valuable for monitoring apoptotic pathway activation. It is widely used in apoptosis research, mitochondrial cell death studies, oncology drug screening, and biochemical or cellular assays requiring quantitative detection of caspase activation and programmed cell death signaling.