Influenza NP (147-155)

Product Name: Influenza NP (147-155)

Sequence One Letter Code: TYQRTRALV

Sequence Three Letter Code: H-Thr-Tyr-Gln-Arg-Thr-Arg-Ala-Leu-Val-OH

Chemical Formula:C48H82N16O14

Molecular Weight: 1107.3

Purity: 95%

Form: Lyophilized

Storage Conditions: - 20 °C

Research Area: Inflammation and Immunology Research

Source / Species: Influenza

Conjugation: Unconjugated

Code Nacres: NA.26

Application: Influenza NP (147–155) is a synthetic nonapeptide epitope derived from the influenza virus nucleoprotein and is presented by the murine MHC class I molecule H-2Kᵈ. It represents a well-characterized CD8⁺ T cell epitope frequently used in mouse models to investigate antigen processing, MHC class I presentation, and antiviral cytotoxic T lymphocyte responses. The peptide is commonly applied in vaccine development studies, immune monitoring assays, and functional analyses of influenza-specific cellular immunity in experimental murine systems.

Current Research: Influenza NP (147–155) is a synthetic nonapeptide epitope derived from the influenza A virus nucleoprotein (NP) and presented in the context of the murine MHC class I molecule H-2Kᵈ. This epitope is one of the best-characterized influenza-specific CD8⁺ T cell determinants in BALB/c and other H-2Kᵈ–restricted mouse models, making it a standard reagent for studying antiviral cellular immunity. As a defined, immunodominant epitope, Influenza NP (147–155) is extensively used to dissect antigen processing, peptide–MHC class I presentation, and cytotoxic T lymphocyte (CTL) responses in controlled murine systems. Immunological Significance The influenza nucleoprotein is an internal viral protein that is highly conserved across influenza A strains. Because NP is abundantly expressed during infection and efficiently processed via the endogenous pathway, NP-derived epitopes are robustly presented on infected cells. The 147–155 epitope is: Naturally processed during influenza infection Efficiently presented by H-2Kᵈ molecules Recognized by influenza-specific CD8⁺ T cells Capable of eliciting strong CTL responses in vivo This makes it a valuable model for evaluating the magnitude, kinetics, and functionality of antiviral T cell immunity. Applications in Murine Immunology 1. Antigen Processing and MHC Class I Presentation Influenza NP (147–155) is used to investigate mechanisms of proteasomal cleavage, TAP-dependent peptide transport, and peptide loading onto H-2Kᵈ. It enables controlled studies of how viral infection, inflammatory signals, or genetic modifications affect antigen presentation. 2. CD8⁺ T Cell Activation and Effector Function The peptide is routinely used for: In vitro restimulation of splenocytes or lymph node cells Intracellular cytokine staining (e.g., IFN-γ, TNF-α) ELISPOT assays Cytotoxicity and killing assays using peptide-pulsed target cells These approaches allow precise quantification of influenza-specific CD8⁺ T cell frequency and function. 3. Vaccine Development and Immunogenicity Studies Because of its reproducible immunodominance, NP (147–155) is incorporated into experimental vaccination platforms to assess: Priming efficiency Memory T cell generation Booster responses Cross-protective immunity It serves as a reliable readout for evaluating adjuvants, viral vectors, and nucleic acid vaccine strategies in H-2Kᵈ–restricted models. 4. Tetramer-Based Immune Monitoring H-2Kᵈ/NP (147–155) tetramers are widely used to track antigen-specific CD8⁺ T cells during infection, vaccination, or adoptive transfer experiments. This facilitates detailed phenotypic and functional characterization of antiviral T cell populations. Experimental Considerations Optimal peptide concentration should be determined empirically depending on assay format and cell source. Inclusion of appropriate negative controls is recommended to account for background activation. As an H-2Kᵈ–restricted epitope, its use is most appropriate in compatible mouse strains (e.g., BALB/c).