LDV FITC

Product Name:LDV FITC

CAS No:1207610-07-8

Purity:95%

Molar Mass:1368.54

Chemical Formula:C69H81N11O17S

Storage:Store at -20 degrees Celsius

Sequence:LDVPAAK

Target:α4β1 integrin

Application:

LDV FITC is a fluorescently labeled peptide, where the LDV sequence (Leu-Asp-Val) is conjugated to FITC (Fluorescein isothiocyanate), a fluorescent dye. The LDV sequence is known for its role in targeting integrins, particularly the α4β1 integrin (VLA-4), which is involved in cell adhesion and migration, especially in immune cells. By attaching FITC to the LDV peptide, researchers can visualize and track the binding interactions of LDV with integrins in various cell types under fluorescent microscopy. LDV FITC is widely used in studies focusing on cell migration, immune cell trafficking, and integrin-related signaling, as well as in assays to investigate integrin-targeted therapeutics.

Current Research:

LDV-FITC is a fluorescently labeled peptide designed to target the α₄β₁ integrin, also known as very late antigen-4 (VLA-4). This peptide has become an essential tool in research focusing on integrin-mediated cell adhesion and signaling, due to its high specificity and fluorescence-based detection capabilities.

Binding Affinity

LDV-FITC binds to the α₄β₁ integrin with high affinity, demonstrating dissociation constant (K_d) values of approximately 0.3 nM in the presence of Mn²⁺ ions and 12 nM in their absence. This strong interaction makes it ideal for studying integrin activity in various cellular environments, including immune cell models like U937 cells.

Applications in Research

LDV-FITC is widely utilized for its versatility in studying integrin expression and function:

Flow Cytometry: Used to quantify and analyze the expression levels of α₄β₁ integrin on the surface of various cell types.
Fluorescence Microscopy: Enables visualization of integrin localization, trafficking, and dynamic interactions in living or fixed cells.
Integrin Conformational Studies: Serves as a valuable tool for assessing the conformational states and activation-dependent changes in α₄β₁ integrin.
Significance of α₄β₁ Integrin

The α₄β₁ integrin is critical in immune cell adhesion and migration, playing pivotal roles in inflammation, immune surveillance, and hematopoiesis. Dysregulation of this integrin is implicated in several diseases, including autoimmune disorders, cancer metastasis, and inflammatory conditions. By probing the interactions and functions of α₄β₁, LDV-FITC contributes to a deeper understanding of its biological significance.

Conclusion

LDV-FITC is a powerful research tool for exploring the biological and pathological roles of the α₄β₁ integrin. Its specificity and fluorescence properties make it indispensable in cell adhesion and integrin-related studies.

Reference:

Chan, J. R., & Cybulsky, M. I. (2003). Detection of High-Affinity α4-Integrin Upon Leukocyte Stimulation by Chemoattractants or Chemokines. Cell Migration in Inflammation and Immunity: Methods and Protocols, 261-268.