NoxA1ds

Product Name:NoxA1ds

CAS No:1435893-78-9

Purity:95%

Molar Mass:1125.32

Chemical Formula:C50H88N14O15

Storage:Store at -20 degrees Celsius

Sequence:EPVDALGKAKV-NH2

Target:NOX1

Application:NoxA1ds is a selective inhibitor of the NADPH oxidase 1 (Nox1) enzyme, a member of the NADPH oxidase family involved in generating reactive oxygen species (ROS). Nox1 plays a significant role in cellular processes like cell proliferation, migration, and oxidative stress, particularly in the context of inflammation and cancer. By inhibiting Nox1, NoxA1ds reduces ROS production, which can be beneficial in studying the effects of oxidative stress on various diseases, including cancer, cardiovascular conditions, and inflammatory disorders. This inhibitor is a valuable tool for exploring therapeutic strategies aimed at modulating ROS-related pathways and preventing oxidative damage in pathological conditions.

Current Research:

NoxA1ds is a synthetic peptide that functions as a highly efficacious and selective inhibitor of NADPH oxidase isoform 1 (Nox1). By mimicking a critical region of the Nox1 activator subunit, NOXA1, NoxA1ds disrupts the essential interaction between Nox1 and NOXA1, thereby inhibiting the enzyme's activation and subsequent reactive oxygen species (ROS) production.
Mechanism of Action
NoxA1ds is designed to replicate specific conserved and non-conserved regions of the putative activation domain of NOXA1. This design enables the peptide to bind to Nox1, effectively blocking the interaction site required for Nox1-NOXA1 binding and enzyme activation. As a result, NoxA1ds selectively inhibits Nox1-derived superoxide anion (O??) production without affecting other NADPH oxidase isoforms such as Nox2, Nox4, or Nox5.
Selectivity and Efficacy
Studies have demonstrated that NoxA1ds exhibits potent inhibitory effects on Nox1 activity with an IC?? of 20 nM. Importantly, it shows high selectivity for Nox1 over other oxidase isoforms and enzymes like xanthine oxidase, ensuring targeted inhibition of Nox1-mediated ROS production.
Research Applications
Due to its specificity, NoxA1ds serves as a valuable tool in biomedical research for:
Investigating Nox1 Function: Elucidating the role of Nox1 in physiological and pathological processes, including its contribution to oxidative stress and cellular signaling.
Disease Modeling: Studying conditions where Nox1-derived ROS are implicated, such as hypertension, atherosclerosis, and certain cancers.
Therapeutic Development: Providing a framework for designing new therapeutic agents targeting Nox1 in diseases associated with excessive ROS production.
Conclusion
NoxA1ds represents a significant advancement in the selective inhibition of Nox1, offering researchers a precise tool to dissect the enzyme's role in health and disease. Its high efficacy and selectivity make it instrumental in advancing our understanding of Nox1-related pathologies and in the development of targeted therapeutic strategies.

Reference:

Ranayhossaini, D. J., Rodriguez, A. I., Sahoo, S., Chen, B. B., Mallampalli, R. K., Kelley, E. E., ... & Pagano, P. J. (2013). Selective recapitulation of conserved and nonconserved regions of putative NOXA1 protein activation domain confers isoform-specific inhibition of Nox1 oxidase and attenuation of endothelial cell migration. Journal of Biological Chemistry, 288(51), 36437-36450.