Dynamin inhibitory peptide

Product Name:Dynamin inhibitory peptide

Synonyms:HY-P1083

CAS No:251634-21-6

Purity:95%

Molar Mass:1121.3

Chemical Formula:C47H80N18O14

Storage:Store at -20 degrees Celsius

Sequence:QVPSRPNRAP

Application:

Dynamin Inhibitory Peptide is a synthetic peptide specifically designed to inhibit the GTPase activity of dynamin, a crucial protein involved in endocytosis and vesicle trafficking. By blocking dynamin function, this peptide is essential for studying the mechanisms of clathrin-mediated endocytosis and its role in various cellular processes, including synaptic transmission and receptor internalization. Dynamin Inhibitory Peptide is highly valuable in research exploring the regulation of membrane dynamics and the development of potential therapeutics targeting these pathways. Its high purity and stability ensure consistent and reliable results in experimental applications.

Current Research:

Dynamin inhibitory peptide (CAS: 251634-21-6) is a synthetic decapeptide with the sequence H₂N-Gln-Val-Pro-Ser-Arg-Pro-Asn-Arg-Ala-Pro-OH, designed to block the interaction between dynamin and amphiphysin. Dynamin, a GTPase, plays a crucial role in clathrin-mediated endocytosis by regulating membrane scission events. This peptide acts as a competitive inhibitor, disrupting dynamin’s binding to amphiphysin, thereby inhibiting its function in vesicle formation and trafficking.

Research employing this peptide has provided significant insights into the molecular mechanisms underlying endocytosis. In neuronal systems, the dynamin inhibitory peptide is particularly valuable for studying the internalization and recycling of neurotransmitter receptors. Notably, it has been shown to inhibit the internalization of GABA receptors, leading to an increase in the amplitude and frequency of miniature inhibitory postsynaptic currents (mIPSCs). These findings highlight the peptide’s role in modulating synaptic transmission, with implications for understanding synaptic plasticity and receptor dynamics.

The utility of this peptide extends beyond neuronal systems. It has been applied in studies exploring endocytic processes in various cell types, including epithelial and immune cells. By blocking dynamin-mediated vesicle trafficking, researchers can investigate how disruptions in endocytosis affect cellular signaling, nutrient uptake, and pathogen entry, contributing to our understanding of these processes in health and disease.

While the dynamin inhibitory peptide is a powerful tool for experimental applications, it has been discontinued by some suppliers, necessitating independent synthesis or the use of alternative reagents. Despite this limitation, its contribution to research remains invaluable, particularly for studies targeting the regulation of membrane trafficking and receptor internalization.

In conclusion, the dynamin inhibitory peptide is a precise and effective inhibitor of dynamin, enabling researchers to explore the complexities of endocytosis and its implications for cellular and physiological functions. Its applications continue to advance the understanding of membrane dynamics across diverse biological systems.

Reference:

Faelber, K., Posor, Y., Gao, S., Held, M., Roske, Y., Schulze, D., ... & Daumke, O. (2011). Crystal structure of nucleotide-free dynamin. Nature, 477(7366), 556-560.