Pseudo RACK1

Product Name:Pseudo RACK1

Synonyms:Pseudo RACK1

Purity:95%

Molar Mass:3198.8

Chemical Formula:C144H225N43O34S3

Storage:Store at -20 degrees Celsius

Sequence:KKWKMRRNQFWIKIQRC CSVEIWD*

Application:

Pseudo RACK1 is a synthetic peptide designed to mimic the regulatory binding domain of the Receptor for Activated C Kinase 1 (RACK1), a key adaptor protein involved in various signaling pathways. Unlike the native RACK1 peptide, Pseudo RACK1 lacks the ability to fully activate protein kinase C (PKC), making it an essential tool for studying the modulation of PKC-mediated signaling without triggering full activation. This peptide is particularly valuable in research focused on dissecting the roles of RACK1 in cellular processes such as proliferation, differentiation, and apoptosis. With its high purity and stability, Pseudo RACK1 provides reliable results in experimental setups.

Current Research:

Pseudo RACK1 is a synthetic peptide designed to activate protein kinase C (PKC) by mimicking the receptor for activated C kinase 1 (RACK1), a scaffolding protein that facilitates PKC localization and function. This peptide comprises a segment derived from the C2 domain of PKC??, linked via a disulfide bridge to the Antennapedia homeodomain vector peptide, which enhances its cell permeability. Upon cellular entry, intracellular reduction of the disulfide bond releases the active peptide, enabling it to interact with PKC.
The primary mechanism of action for Pseudo RACK1 involves binding to PKC, thereby promoting its activation in the absence of traditional activators. This interaction effectively simulates the natural anchoring function of RACK1, facilitating PKC's translocation to specific subcellular sites and subsequent phosphorylation of target substrates.
In experimental settings, Pseudo RACK1 has been utilized to dissect PKC-mediated signaling pathways. For instance, studies have demonstrated that treatment with Pseudo RACK1 leads to rapid activation of PKC??, resulting in the phosphorylation of downstream targets such as heat shock protein 27 (Hsp27). This phosphorylation event has been linked to enhanced immune cell activation, as evidenced by increased cytokine production and upregulation of surface markers like CD86.
Despite its utility in research, the commercial availability of Pseudo RACK1 has become limited, with some suppliers discontinuing its sale for unspecified reasons. Researchers interested in employing this peptide may need to explore alternative sources or consider custom synthesis to obtain it for their studies.
In summary, Pseudo RACK1 serves as a valuable tool for investigating PKC-related signaling mechanisms. By selectively activating PKC isoforms, it enables researchers to elucidate the roles of these kinases in various cellular processes, thereby contributing to a deeper understanding of cell signaling dynamics.

Reference:

Corsini, E., Pinto, A., Galbiati, V., Viviani, B., Galli, C. L., Marinovich, M., & Racchi, M. (2014). Corticosteroids modulate the expression of the PKC-anchoring protein RACK-1 and cytokine release in THP-1 cells. Pharmacological Research, 81, 10-16.