RETF-4NA

Product Name:RETF-4NA

CAS No:1160928-63-1

Purity:95%

Molar Mass:713.74

Chemical Formula:C32H43N9O10

Storage:Store at -20 degrees Celsius

Sequence:RETF

Application:

RETF-4NA is a research peptide commonly used in studies related to receptor binding and signaling pathways. This peptide's structure and composition make it suitable for investigating interactions with specific receptors, aiding in understanding cellular processes and receptor-mediated responses. It is often employed in pharmacological and biochemical experiments to elucidate mechanisms of action within these pathways. RETF-4NA is valuable for advancing receptor-targeted drug development and understanding receptor dynamics in various biological systems. This peptide is typically utilized in controlled experimental settings for high-precision research outcomes.

Current Research:

RETF-4NA, also known as acetyl-L-Arg-Glu-Thr-Phe-4-nitroanilide, is a synthetic tetrapeptide substrate specifically designed to assess chymase activity. Chymase, a serine protease predominantly found in mast cells, plays a crucial role in various physiological and pathological processes, including inflammation and tissue remodeling. Accurate measurement of chymase activity is essential for understanding its function in health and disease.
Specificity and Sensitivity
RETF-4NA exhibits high specificity and sensitivity toward chymase. Studies have demonstrated that it is selectively hydrolyzed by chymase, with minimal or no cleavage by other proteases such as cathepsin G and chymotrypsin. This selectivity is particularly advantageous when analyzing complex biological samples where multiple proteases may be present.
Applications in Research
The substrate is widely utilized in biochemical assays to quantify chymase activity in various biological samples, including tissue extracts, cell cultures, and bodily fluids like serum. Its application extends to research areas focusing on cardiovascular diseases, allergic reactions, and fibrotic conditions, where chymase activity is implicated. For instance, RETF-4NA has been employed to detect active chymase in serum, aiding in the study of mast cell-related diseases such as mastocytosis.
Advantages
One of the notable advantages of RETF-4NA is its ability to detect chymase activity even when the enzyme is bound to ???-macroglobulin (???M), a plasma protein that can inhibit proteases. This feature enhances the detection sensitivity in serum-based assays, as ???M-bound chymase retains its activity toward RETF-4NA, facilitating more accurate measurements.
Conclusion
RETF-4NA serves as a valuable tool in the study of chymase activity, providing researchers with a reliable and specific substrate for their investigations. Its high selectivity and sensitivity make it indispensable in advancing our understanding of chymase's role in various physiological and pathological contexts.

Reference:

Zhou, X., Whitworth, H. S., Brown, T. A., Goswami, R., Eren, E., Lucas, J. A., ... & Walls, A. F. (2011). Mast cell chymase: a useful serum marker in anaphylaxis. Journal of Allergy and Clinical Immunology, 127(2), AB143.