TCS 184

Product Name:TCS 184

CAS No:1315378-71-2

Purity:95%

Molar Mass:1425.58

Chemical Formula:C58H96N20O20S

Storage:Store at -20 degrees Celsius

Sequence:TAESTFMRPSGSR

Application:

TCS 184 is a selective small molecule inhibitor designed to target the protein kinase glycogen synthase kinase-3 beta (GSK-3β). By inhibiting GSK-3β, TCS 184 disrupts various cellular processes that this kinase regulates, including glycogen metabolism, cell proliferation, and apoptosis. GSK-3β is involved in multiple signaling pathways, and its dysregulation is associated with diseases such as cancer, diabetes, and neurodegenerative disorders. TCS 184 is commonly used in research to explore the role of GSK-3β in these conditions, providing insights into potential therapeutic approaches that involve modulating GSK-3β activity to treat related diseases.

Current Research:

TCS 184 (CAS: 1315378-71-2) is a synthetic peptide used as a scrambled control in experiments involving TCS 183, a known glycogen synthase kinase-3 (GSK-3) inhibitor. With a randomized sequence that lacks the specific functional motif required for GSK-3 interaction, TCS 184 plays a critical role in distinguishing between specific and nonspecific effects of GSK-3 inhibition.

Structure and Properties

The peptide has the amino acid sequence Thr-Ala-Glu-Ser-Thr-Phe-Met-Arg-Pro-Ser-Gly-Ser-Arg, with modifications such as C-terminal amidation to enhance its stability. TCS 184 is water-soluble (up to 1 mg/mL) and stable when stored at -20°C under desiccated conditions, ensuring its suitability for diverse experimental applications.

Function and Mechanism

As a scrambled control, TCS 184 does not bind or inhibit GSK-3. This distinguishes it from TCS 183, which specifically blocks GSK-3 activity by interfering with its phosphorylation mechanisms. By lacking functional activity, TCS 184 ensures that any observed cellular or biochemical changes in comparative studies are due to TCS 183's direct inhibition of GSK-3 and not off-target or nonspecific effects.

Applications in Research

TCS 184 is extensively used in molecular and biochemical studies focusing on GSK-3-mediated pathways. It helps validate the role of GSK-3 in processes such as glycogen metabolism, gene expression, neurodegeneration, and cellular signaling. By providing an inactive control, it strengthens experimental reliability and supports the specificity of conclusions.

Conclusion

TCS 184 is an indispensable tool in GSK-3-related research. Its use as a control peptide ensures the accuracy of findings and enhances the robustness of studies exploring GSK-3’s roles in health and disease, particularly in metabolic and neurodegenerative disorders. Its design emphasizes the importance of specificity in targeted molecular studies.

Reference:

Peineau, S., Taghibiglou, C., Bradley, C., Wong, T. P., Liu, L., Lu, J., ... & Collingridge, G. L. (2007). LTP inhibits LTD in the hippocampus via regulation of GSK3??. Neuron, 53(5), 703-717.