Z-Val-Gly-Arg p-nitroanilide acetate salt

Product Name:Z-Val-Gly-Arg p-nitroanilide acetate salt

Synonyms:L-Argininamide, N-[(phenylmethoxy)carbonyl]-L-valylglycyl-N-(4-nitrophenyl)-, monoacetate acetic acid;benzyl N-[(2S)-1-[[2-[[(2S)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-3-methyl-1-oxobutan-2-yl]carbamate

CAS No:86170-43-6

Purity:95%

Molar Mass:644.7

Chemical Formula:C29H40N8O9

Storage:Store at -20 degrees Celsius

Application:

Z-Val-Gly-Arg p-nitroanilide acetate salt is a synthetic peptide substrate commonly used in enzymatic assays to study protease activity, particularly that of serine proteases such as trypsin and thrombin. The Z (benzyloxycarbonyl) group protects the N-terminal of the peptide, while the p-nitroanilide serves as a chromogenic group that releases a yellow color upon cleavage by the target enzyme. This colorimetric change allows for the quantification of enzyme kinetics and inhibitor efficacy in biochemical and pharmacological research. Z-Val-Gly-Arg p-nitroanilide acetate salt is essential for studying enzyme-substrate interactions and screening potential therapeutic inhibitors, making it a critical tool in drug development and enzymology.

Current Research:

Z-Val-Gly-Arg p-nitroanilide acetate salt (CAS: 86170-43-6) is a chromogenic peptide substrate widely used in enzymatic assays to evaluate the activity of serine proteases, including trypsin and urokinase. Its structure comprises a tripeptide sequence, N-benzyloxycarbonyl-valyl-glycyl-arginine, conjugated to a p-nitroaniline (pNA) group. When cleaved by a target protease at the arginine-pNA bond, it releases free p-nitroaniline, which can be quantitatively detected via absorbance at 405 nm. This enables precise measurement of enzyme kinetics and inhibitor effects in a variety of biochemical contexts.

This compound is particularly valued in high-throughput screening applications to identify protease inhibitors, a critical step in drug discovery. Its specificity for certain proteases makes it an indispensable tool for characterizing enzymatic mechanisms and exploring proteolytic pathways in physiological and pathological processes.

Z-Val-Gly-Arg p-nitroanilide acetate salt offers excellent stability and reliable performance when stored under appropriate conditions, such as at −20°C. Its use spans basic research, including detailed studies of enzyme-substrate interactions, to applied science, aiding in the development of therapeutic agents targeting protease-mediated diseases.

In summary, Z-Val-Gly-Arg p-nitroanilide acetate salt is a pivotal reagent for advancing our understanding of proteolytic systems and for facilitating the innovation of targeted inhibitors for clinical applications.

Reference:

Matsuura, K., Yamamoto, K., & Sinohara, H. (1994). Amidase activity of human Bence Jones proteins. Biochemical and biophysical research communications, 204(1), 57-62.