Caspase 8 Substrate 1, chromogenic

Product Name: Caspase 8 Substrate 1, chromogenic

Sequence One Letter Code: Ac-IETD-pNA

Sequence Three Letter Code: Ac - Ile-Glu-Thr-Asp - pNA

Cas No: 219138-21-3

Chemical Formula:C27H38N6O12

Molecular Weight: 638.7

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

SMILES: CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C

IUPAC: (4S)-4-[[(2S,3S)-2-acetamido-3-methylpentanoyl]amino]-5-[[(2S,3R)-1-[[(2S)-3-carboxy-1-(4-nitroanilino)-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-5-oxopentanoic acid

INCHIKEY: FHURKTIGZAIQGR-BQCLNCLCSA-N

INCHI:

InChI=1S/C27H38N6O12/c1-5-13(2)22(28-15(4)35)26(42)30-18(10-11-20(36)37)24(40)32-23(14(3)34)27(43)31-19(12-21(38)39)25(41)29-16-6-8-17(9-7-16)33(44)45/h6-9,13-14,18-19,22-23,34H,5,10-12H2,1-4H3,(H,28,35)(H,29,41)(H,30,42)(H,31,43)(H,32,40)(H,36,37)(H,38,39)/t13-,14+,18-,19-,22-,23-/m0/s1

Conjugation: Conjugated

Conjugation Type: Conjugates

Code Nacres: NA.26

Application: Caspase-8 Chromogenic Substrate is a pNA-derived peptide substrate designed for measuring caspase-8 activity, a central initiator enzyme in extrinsic apoptosis signaling. This substrate is also recognized by granzyme B, supporting its use in immune-mediated cytotoxicity research. Upon enzymatic cleavage, p-nitroaniline is released, generating a colorimetric signal measurable at approximately 405 nm. Caspase-8 Chromogenic Substrate is widely used in apoptosis assays, immune signaling studies, programmed cell death research, drug discovery workflows, and screening of compounds targeting caspase activation, death receptor pathways, and cytotoxic lymphocyte-mediated cell death.