CFP10 (71–85)

Product Name: CFP10 (71–85)

Sequence One Letter Code: EISTNIRQAGVQYSR

Sequence Three Letter Code: H-Glu-Ile-Ser-Thr-Asn-Ile-Arg-Gln-Ala-Gly-Val-Gln-Tyr-Ser-Arg-OH

Chemical Formula:C72H120N24O25

Molecular Weight: 1722

Purity: 95%

Form: Lyophilized

Storage Conditions: - 20 °C

Research Area: Inflammation and Immunology Research

Source / Species: mycobacterium

Conjugation: Unconjugated

Code Nacres: NA.26

Application: CFP10 (71–85) is a synthetic 15-amino acid peptide derived from the culture filtrate protein 10 of Mycobacterium tuberculosis, a major secreted antigen associated with tuberculosis infection. CFP10 is strongly immunogenic and stimulates interferon-γ production and cytotoxic T lymphocyte responses in both CD4⁺ and CD8⁺ T cells across diverse MHC backgrounds. This peptide fragment contains defined T cell epitopes that contribute to robust cellular immune activation. CFP10 (71–85) is widely used in studies of antigen processing, MHC-restricted presentation, and T cell recognition mechanisms. It supports evaluation of host–pathogen interactions, immune monitoring assays, and vaccine research focused on tuberculosis. By providing a reproducible antigenic stimulus, this peptide facilitates investigation of cellular immunity, T cell functionality, and protective immune responses in both basic immunology and translational infectious disease research settings.

Current Research: CFP10 (71–85) is a synthetic 15-amino acid peptide derived from culture filtrate protein 10 (CFP10) of Mycobacterium tuberculosis, one of the most immunodominant antigens associated with tuberculosis (TB) infection. CFP10 is a secreted virulence factor encoded within the RD1 genomic region and forms a functional complex with ESAT-6, contributing to mycobacterial pathogenicity and host immune modulation. Because CFP10 is absent from Bacillus Calmette–Guérin (BCG) vaccine strains and most non-tuberculous mycobacteria, it is highly relevant for TB-specific immune investigations. The 71–85 fragment contains defined T cell epitopes that elicit strong cellular immune responses. Studies have demonstrated that CFP10-derived peptides stimulate interferon-γ (IFN-γ) production and activate cytotoxic T lymphocytes across diverse MHC class I and class II backgrounds. This broad immunogenicity makes CFP10 (71–85) a valuable reagent for examining antigen-specific CD4⁺ and CD8⁺ T cell responses in infected or exposed individuals. In antigen presentation research, CFP10 (71–85) is used to evaluate MHC-restricted peptide loading and T cell receptor recognition. By pulsing antigen-presenting cells with the defined peptide, investigators can bypass upstream antigen processing steps and directly assess peptide–MHC stability and T cell activation thresholds. This controlled system enables mechanistic studies of T cell specificity, functional avidity, and clonal expansion. The peptide is widely applied in ex vivo immune monitoring assays using peripheral blood mononuclear cells from individuals with latent or active tuberculosis. Upon stimulation, CFP10-specific T cells can be assessed for cytokine production, including IFN-γ and tumor necrosis factor-α, as well as proliferation and cytolytic function. Common assay platforms include ELISpot, intracellular cytokine staining, flow cytometry–based activation marker analysis, and cytotoxicity assays. These applications support quantitative assessment of TB-specific cellular immunity. CFP10 (71–85) is also relevant in vaccine development research. Because protective immunity against tuberculosis relies heavily on robust T cell responses, defined antigenic peptides are essential for evaluating vaccine-induced cellular activation. The peptide enables standardized comparison of immune responses across preclinical models and clinical studies, supporting assessment of immunogenicity and durability. Beyond immune monitoring, CFP10-derived peptides are used to investigate host–pathogen interactions and immune regulation mechanisms. Research focusing on T cell differentiation, memory formation, and exhaustion frequently incorporates CFP10 epitopes to analyze how chronic antigen exposure influences functional capacity. Such studies contribute to understanding immune control of persistent intracellular pathogens. The defined sequence and reproducible immunological activity of CFP10 (71–85) make it particularly useful for dissecting mechanisms of T cell–mediated protection. By providing a consistent and TB-specific antigenic stimulus, the peptide supports comparative studies of immune competence in different populations, including immunocompromised individuals and vaccine recipients. Overall, CFP10 (71–85) is a well-characterized and immunologically relevant peptide that facilitates investigation of antigen processing, MHC-restricted presentation, and T cell recognition in tuberculosis research. Its application spans basic immunology, translational infectious disease studies, immune monitoring, and vaccine evaluation, contributing to a deeper understanding of cellular immunity against Mycobacterium tuberculosis.