Crosstide [GRPRTSSFAEG], 5-FAM labeled

Product Name: Crosstide [GRPRTSSFAEG], 5-FAM labeled

Sequence One Letter Code: 5-FAM-GRPRTSSFAEG

Sequence Three Letter Code: 5-FAM-Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly-OH

Chemical Formula:C69H87N17O23

Molecular Weight: 1522.6

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

Source / Species: Drosophila

Conjugation: Conjugated

Conjugation Type: Fluorescent dyes

Code Nacres: NA.26

Application: Crosstide is a synthetic peptide substrate derived from glycogen synthase kinase-3 and widely recognized as a target sequence for Akt/PKB activity. This 5-FAM-labeled fluorescent peptide enables sensitive detection and quantitative monitoring of kinase-mediated phosphorylation in biochemical assays. Crosstide is efficiently phosphorylated by Akt/PKB isoforms and related kinases, including MAPKAP kinase-1 and p70S6 kinase. Its fluorescent labeling makes it suitable for real-time kinase assays, enzyme kinetic analysis, and high-throughput screening platforms. Crosstide is commonly used in signal transduction research, especially for studying Akt pathway activation, kinase specificity, and phosphorylation-dependent cellular regulation.