[Glu27]-PKC (19-36)

Product Name:[Glu27]-PKC (19-36)

CAS No:309247-49-2

Purity:95%

Molar Mass:2124.43

Chemical Formula:C92H154N32O26

Storage:Store at -20 degrees Celsius

Sequence:RFARKGALEQKNVHEVKN

Target:protein kinase C

Application:

[Glu27]-PKC (19-36) is a selective protein kinase C (PKC) inhibitor derived from the pseudosubstrate region of PKC. This synthetic peptide, featuring a Glutamic acid substitution at position 27, acts by competitively inhibiting PKC activation. PKC plays a pivotal role in various cellular processes, including cell growth, differentiation, and apoptosis. [Glu27]-PKC (19-36) is commonly used in research to investigate PKC-mediated signaling pathways, particularly in the study of cancer, inflammation, and cardiovascular diseases. By blocking PKC activity, this peptide provides insight into therapeutic approaches targeting PKC-related pathways in a wide range of pathological conditions.

Current Research:

[Glu²⁷]-Protein Kinase C (19-36), commonly referred to as [Glu²⁷]-PKC (19-36), is an 18-amino acid peptide fragment derived from the regulatory domain of Protein Kinase C (PKC). This peptide includes a substitution at position 27, where glutamic acid (Glu) replaces the native amino acid, potentially altering its functional properties.

Structural Composition

The amino acid sequence of [Glu²⁷]-PKC (19-36) is:

H₂N-Arg-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Glu-Gln-Lys-Asn-Val-His-Glu-Val-Lys-Asn-OH

This sequence corresponds to residues 19 through 36 of the PKC protein, with the Glu substitution at position 27.

Functional Significance

PKC is a family of serine/threonine kinases involved in various cellular processes, including signal transduction, proliferation, and differentiation. The regulatory domain of PKC contains regions critical for its activation and interaction with other cellular components. Peptides derived from this domain, such as [Glu²⁷]-PKC (19-36), are utilized in research to study PKC's regulatory mechanisms and to develop inhibitors that modulate its activity.

Applications in Research

The [Glu²⁷]-PKC (19-36) peptide serves as a tool in biochemical and pharmacological studies to:

Investigate the structural and functional aspects of PKC activation.

Develop and assess potential PKC inhibitors for therapeutic purposes.

Explore the role of PKC in various signaling pathways and disease states.

Conclusion

[Glu²⁷]-PKC (19-36) is a valuable peptide fragment for researchers studying the regulatory mechanisms of Protein Kinase C. Its specific sequence and structural properties make it a useful tool in elucidating PKC's role in cellular signaling and in the development of targeted therapeutic agents.

Reference:

Boehm, S., Huck, S., & Freissmuth, M. (1996). Involvement of a phorbol ester-insensitive protein kinase C in the α2-adrenergic inhibition of voltage-gated calcium current in chick sympathetic neurons. Journal of Neuroscience, 16(15), 4596-4603.