HCV NS3/4A Protease Substrate

Product Name: HCV NS3/4A Protease Substrate

Sequence One Letter Code: Ac-DE-Dap(QXL®520)-EE-Abu-ψ-[COO]AS-C(5-FAMsp)-NH2

Sequence Three Letter Code: Ac-Asp-Glu-Dap(QXL®520)-Glu-Glu-Abu-ψ-[COO]-Ala -Ser-Cys(5-FAMsp)-NH2

Molecular Weight: 1913.8

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C

Source / Species: HCV

Conjugation: Unconjugated

Code Nacres: NA.26

Application: HCV NS3/4A Protease FRET Substrate is a highly sensitive fluorogenic peptide substrate developed for measuring Hepatitis C virus NS3/4A protease activity. NS3/4A is essential for viral polyprotein processing and replication, making it an important antiviral drug target. This FRET substrate enables detection of protease activity at sub-picomole levels, with fluorescence monitored at Abs/Em 490/512 nm after enzymatic cleavage. It is widely used in enzymology research, antiviral inhibitor screening, protease kinetics, mechanistic studies of HCV replication, and therapeutic development programs targeting viral protease function.