[Lys(Me2)36]-Histone H3 (21-44)-GK(Biotin)

Product Name: [Lys(Me2)36]-Histone H3 (21-44)-GK(Biotin)

Sequence One Letter Code: ATKAARKSAPATGGV-K(Me2)-KPHRYRPG-GK(Biotin)

Sequence Three Letter Code: H-Ala-Thr-Lys-Ala-Ala-Arg-Lys-Ser-Ala-Pro-Ala-Thr-Gly-Gly-Val-Lys(Me2)-Lys-Pro-His-Arg-Tyr-Arg-Pro-Gly-Gly-Lys(Biotin)-OH

Molecular Weight: 2945.6

Purity: 95%

Form: Lyophilized

Storage Conditions: - 20 °C

Research Area: epigenetics

Source / Species: human

Conjugation: Conjugated

Conjugation Type: Biotins

Code Nacres: NA.26

Application: [Lys(Me2)36]-Histone H3 (21–44)-GK(Biotin) is a synthetic histone peptide corresponding to residues 21–44 of histone H3, featuring dimethylation at lysine 36 and a C-terminal biotinylated lysine. H3K36me2 is an epigenetic modification involved in chromatin regulation and transcriptional control, including recruitment of histone deacetylase complexes. The biotin tag enables affinity-based assays such as pull-down and binding studies. This peptide is widely used in epigenetics research to investigate histone modification recognition, chromatin-associated enzyme activity, and demethylation processes, particularly those mediated by Jumonji C-domain proteins, supporting studies of gene regulation and chromatin dynamics.

Current Research: [Lys(Me2)36]-Histone H3 (21–44)-GK(Biotin) is a synthetic histone peptide spanning residues 21–44 of histone H3, featuring site-specific dimethylation at lysine 36 (H3K36me2) and a C-terminal glycine–lysine–biotin modification. This design combines a biologically relevant epigenetic mark with an affinity tag, making it a powerful tool for investigating chromatin regulation, enzyme specificity, and histone–protein interactions. H3K36me2 and Its Role in Chromatin Biology Dimethylation at lysine 36 of histone H3 (H3K36me2) is a key epigenetic modification associated with: Transcriptional regulation across gene bodies Chromatin organization and accessibility Recruitment of chromatin-modifying complexes, including histone deacetylases (HDACs) Maintenance of genomic stability Unlike H3K36me3, which is enriched in actively transcribed regions, H3K36me2 often occupies broader genomic domains and plays a role in fine-tuning transcriptional output and chromatin structure. Structural Design and Functional Features This peptide incorporates two important elements: Site-specific H3K36 dimethylation, enabling precise study of this modification C-terminal GK(Biotin) tag, allowing affinity-based applications The biotin moiety provides strong binding to streptavidin or avidin, facilitating: Efficient immobilization on assay platforms Selective capture of interacting proteins Sensitive detection in biochemical workflows The positioning of the biotin tag ensures minimal interference with the modified lysine and surrounding recognition motifs. Applications in Histone Modification and Enzyme Studies [Lys(Me2)36]-Histone H3 (21–44)-GK(Biotin) is widely used as a defined substrate for studying enzymes involved in histone methylation dynamics. Key applications include: Histone methyltransferase (HMT) studies examining K36-specific activity Histone demethylase (KDM) assays, particularly for Jumonji C (JmjC)-domain proteins Analysis of modification turnover and enzymatic specificity Investigation of cross-talk between histone marks These studies help elucidate how H3K36 methylation is established, maintained, and reversed. Protein Interaction and Pull-Down Assays The biotinylated peptide is highly suitable for protein–histone interaction studies. Immobilization on streptavidin matrices enables: Pull-down assays to isolate H3K36me2-binding proteins Identification of chromatin reader domains that recognize dimethylated lysines Characterization of HDAC-containing complexes recruited by H3K36me2 Proteomic analysis of chromatin-associated factors Such experiments are essential for understanding how epigenetic marks are interpreted within the cell. Role in Chromatin Regulation and Gene Expression H3K36me2 is implicated in regulating transcriptional elongation, chromatin compaction, and epigenetic stability. This peptide enables controlled investigation of: Recruitment of repressive or regulatory complexes Coordination between methylation and deacetylation pathways Dynamic changes in chromatin state during development and differentiation It also supports studies of how dysregulated H3K36 methylation contributes to disease states, including cancer. Applications in Epigenetics and Disease Research Alterations in H3K36 methylation are associated with: Cancer-related mutations in methyltransferases and demethylases Epigenetic reprogramming and transcriptional dysregulation Defects in chromatin organization and genome integrity This peptide is therefore used in: Screening inhibitors of histone-modifying enzymes Functional studies of JmjC-domain demethylases Investigation of epigenetic therapeutic targets A Versatile Tool for Studying Histone Methylation Dynamics [Lys(Me2)36]-Histone H3 (21–44)-GK(Biotin) provides a precise and experimentally versatile model for analyzing H3K36 dimethylation. By combining a biologically relevant modification with an affinity handle, it supports detailed studies of enzyme activity, protein recognition, and chromatin signaling pathways. Its application continues to advance understanding of epigenetic regulation, transcriptional control, and the molecular mechanisms governing chromatin dynamics in health and disease.