Neuropeptide Y (scrambled)

Product Name:Neuropeptide Y (scrambled)

Purity:95%

Molar Mass:4253.7

Chemical Formula:C190H287N55O57

Storage:Store at -20 degrees Celsius

Sequence:SKPQRDANREPTRYAIYDYSNPDIELHYLRPAYALG-NH2

Application:

Neuropeptide Y (scrambled) is a synthetic peptide with a random sequence arrangement compared to the native Neuropeptide Y (NPY). It is commonly used as a negative control in experiments to assess the specificity of NPY-related biological activities. NPY is involved in various physiological processes such as appetite regulation, stress response, and energy homeostasis. The scrambled version does not exhibit the typical receptor-binding properties of the native peptide, making it useful in validating the specific effects of NPY in studies related to neurobiology, metabolism, and cardiovascular research.

Current Research:

Scrambled Neuropeptide Y (NPY) is a control peptide used in experimental studies to validate the specificity of NPY’s biological effects. It retains the same amino acid composition as the functional NPY peptide but lacks the native sequence required for receptor binding. This design ensures it does not interact with NPY receptors (Y1, Y2, Y4, and Y5), serving as a negative control in experiments investigating NPY’s role in physiological and pathological processes.

Applications in Research
Receptor Binding Specificity
Scrambled NPY is commonly employed to confirm that observed biological effects are directly mediated by NPY binding to its receptors. For instance, in studies of appetite regulation, NPY is known to stimulate feeding behavior via the Y1 receptor. Scrambled NPY helps demonstrate that this effect is specific and not due to nonspecific interactions.

Neurobiological Studies
In neurodegenerative and psychiatric research, scrambled NPY is used to validate the neuroprotective and anxiolytic effects of native NPY. Its inclusion ensures that findings are attributed to functional receptor-ligand interactions rather than unrelated peptide activities.

Cardiovascular Research
NPY influences vascular tone and blood pressure through its interaction with Y1 and Y2 receptors. Scrambled NPY is used to distinguish receptor-specific effects from off-target responses in cardiovascular studies.

Cancer Research
NPY has been implicated in tumor growth and angiogenesis in some cancers. Scrambled NPY is employed to verify the role of NPY in these processes, ensuring experimental accuracy.

Importance in Experimental Design
The use of scrambled NPY as a control enhances the rigor and validity of experimental studies by eliminating confounding variables. This approach is crucial in developing targeted therapies and understanding NPY’s diverse physiological roles.

Reference:

Rose, J. B., Crews, L., Rockenstein, E., Adame, A., Mante, M., Hersh, L. B., … & Masliah, E. (2009). Neuropeptide Y fragments derived from neprilysin processing are neuroprotective in a transgenic model of Alzheimer's disease. Journal of Neuroscience, 29(4), 1115-1125.