OVA (323-339), amide

Product Name: OVA (323-339), amide

Sequence One Letter Code: ISQAVHAAHAEINEAGR-NH2

Sequence Three Letter Code: H-Ile-Ser-Gln-Ala-Val-His-Ala-Ala-His-Ala-Glu-Ile-Asn-Glu-Ala-Gly-Arg-NH2

Chemical Formula:C74H121N27O24

Molecular Weight: 1773.1

Purity: 95%

Form: Lyophilized

Storage Conditions: - 20 °C

Research Area: Inflammation and Immunology Research

Source / Species: chicken

Conjugation: Unconjugated

Code Nacres: NA.26

Application: OVA (323–339), amide is a synthetic peptide corresponding to residues 323–339 of ovalbumin and represents a well-characterized MHC class II–restricted epitope. This fragment contains multiple CD4⁺ T-cell epitopes and can be presented by I-Aᵈ molecules in different binding registers, with residues 327–333 playing a key role in MHC interaction. It is widely used in immunology research to study antigen presentation, T-cell activation, and helper T-cell responses. The peptide supports investigations into immune recognition, epitope mapping, and adaptive immune signaling, making it a standard model for studying CD4⁺ T-cell–mediated immune responses.

Current Research: OVA (323–339), amide is a synthetic peptide derived from ovalbumin, corresponding to amino acid residues 323–339. It represents a well-characterized MHC class II–restricted epitope, widely used in immunology as a model antigen for studying CD4⁺ T-cell responses, antigen presentation, and adaptive immunity. The C-terminal amidation enhances peptide stability while preserving its biological function in immune recognition. MHC Class II Presentation and Epitope Features This peptide is presented by MHC class II molecules, particularly I-Aᵈ, enabling recognition by CD4⁺ helper T cells. A key feature of OVA (323–339) is its ability to bind MHC molecules in multiple registers, meaning the peptide can align differently within the binding groove. Critical aspects include: Core binding region (residues 327–333) essential for MHC interaction Flexible binding registers, allowing diverse T-cell receptor (TCR) recognition Multiple overlapping epitopes, supporting robust CD4⁺ T-cell activation This versatility makes it an excellent system for studying peptide–MHC–TCR interactions. Role in CD4⁺ T-Cell Activation Upon presentation by antigen-presenting cells (APCs), OVA (323–339) is recognized by antigen-specific CD4⁺ T cells, leading to: T-cell activation and proliferation Cytokine production (e.g., IL-2, IFN-γ, IL-4 depending on context) Differentiation into helper T-cell subsets (Th1, Th2, etc.) These responses are central to orchestrating adaptive immune responses, including B-cell activation and antibody production. Applications in Immunology Research OVA (323–339), amide is a standard tool in experimental immunology due to its well-defined immunogenic properties. Common applications include: Antigen presentation studies using MHC class II pathways CD4⁺ T-cell activation assays in vitro and in vivo Epitope mapping and binding register analysis TCR specificity and affinity studies Evaluation of helper T-cell differentiation and cytokine profiles Its compatibility with established ovalbumin model systems enhances its utility across diverse experimental platforms. Use in Model Immune Systems Ovalbumin-derived peptides are widely used in model antigen systems, particularly in murine studies. OVA (323–339) is often employed alongside: Transgenic T-cell models (e.g., OVA-specific CD4⁺ T cells) Adoptive transfer experiments Vaccination and tolerance models These systems allow precise investigation of antigen-specific immune responses under controlled conditions. Insights into Antigen Recognition and Immune Regulation Because OVA (323–339) can bind MHC in multiple registers, it is especially valuable for studying: Flexibility of peptide–MHC interactions Diversity of T-cell receptor recognition Mechanisms of immune specificity and cross-reactivity This makes it a powerful model for understanding how the immune system distinguishes between different antigens. Applications in Tolerance and Immune Modulation Studies Beyond activation, this peptide is also used to explore immune tolerance and regulation, including: Induction of antigen-specific tolerance Regulatory T-cell (Treg) responses Mechanisms of immune suppression and modulation Such studies are important for understanding autoimmune diseases and designing immunotherapies. A Standard Tool for CD4⁺ T-Cell Research OVA (323–339), amide remains one of the most widely used MHC class II model epitopes in immunology. Its well-characterized interaction with MHC molecules and T-cell receptors makes it indispensable for studying antigen presentation, helper T-cell activation, and adaptive immune signaling. By enabling detailed analysis of epitope recognition, T-cell responses, and immune regulation, this peptide continues to support advances in understanding immune system function and therapeutic strategies targeting adaptive immunity.