Product Name: Pam3CSK4 Biotin
Purity: 95%
Molar Mass: 1962.8
Chemical Formula: C103H192N14O17S2
Storage: Store at -20 degrees Celsius
Sequence: Pam3C-SKKKK
Target: TLR1
Application:
Pam3CSK4 Biotin is a synthetic lipopeptide that acts as a Toll-like receptor 2 (TLR2) agonist, conjugated with biotin for detection and affinity applications. By binding to TLR2, it activates the innate immune response, leading to the production of pro-inflammatory cytokines. This lipopeptide is widely used in immunology research to study TLR2 signaling pathways, innate immunity, and inflammatory responses. The biotinylation allows for easy detection, purification, and interaction studies using streptavidin-based systems. Pam3CSK4 Biotin is also utilized in vaccine development and adjuvant research to enhance immune responses.
Current Research:
Pam3CSK4-Biotin is a synthetic, biotinylated triacylated lipopeptide that emulates the acylated amino terminus of bacterial lipoproteins. It serves as a potent agonist for the Toll-like receptor 2 (TLR2) and Toll-like receptor 1 (TLR1) heterodimer, effectively activating the pro-inflammatory transcription factor NF-κB. The biotinylation of Pam3CSK4 facilitates its use in various binding assays and detection applications, enhancing its utility in immunological studies.
Mechanism of Action
Pam3CSK4-Biotin engages the TLR2/TLR1 heterodimer on the surface of immune cells, initiating a signaling cascade that culminates in the activation of NF-κB. This activation leads to the transcription of pro-inflammatory cytokines and other mediators, thereby modulating immune responses. The biotin moiety allows for the conjugation of Pam3CSK4 to streptavidin-linked probes, enabling precise detection and quantification in experimental settings.
Applications in Research
The biotinylated form of Pam3CSK4 is particularly advantageous in studies requiring the tracking or immobilization of the ligand. It has been employed in the investigation of TLR2/TLR1-mediated signaling pathways, assessment of receptor-ligand interactions, and evaluation of immune cell activation. Additionally, Pam3CSK4-Biotin is utilized in the development of diagnostic assays and in the study of pathogen-host interactions, providing insights into the innate immune response.
Advantages of Biotinylation
The incorporation of a biotin group into Pam3CSK4 enhances its versatility in laboratory applications. Biotinylation enables the use of streptavidin-based detection systems, which are known for their high affinity and specificity. This modification facilitates the study of molecular interactions and the localization of TLR2/TLR1 engagement within complex biological systems.
Conclusion
Pam3CSK4-Biotin is a valuable tool in immunological research, offering a robust method for activating TLR2/TLR1 pathways while providing the added benefits of biotinylation for detection and assay development. Its application enhances the understanding of innate immune mechanisms and supports the advancement of therapeutic and diagnostic innovations.
Reference:
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