Waglerin-1-FAM

Product Name:Waglerin-1-FAM

Purity:95%

Molar Mass:2878.27

Chemical Formula:C133H185N37O32S2

Storage:Store at -20 degrees Celsius

Sequence:FAM-Gly-Gly-Lys-Pro-Asp-Leu-Arg-Pro-Cys9-His-Pro-Pro-Cys13-His-Tyr-Ile-Pro-Arg-Pro-Lys-Pro-Arg-OH

Application:

Waglerin-1-FAM is a fluorescently labeled version of the peptide toxin Waglerin-1, derived from the venom of the Wagler’s pit viper (Tropidolaemus wagleri). This toxin specifically targets and inhibits nicotinic acetylcholine receptors (nAChRs) in skeletal muscle, particularly the α1 subunit, leading to the blockade of neuromuscular transmission. By conjugating Waglerin-1 with a fluorescent dye like FAM, researchers can visualize and track its binding and interactions with nAChRs in real time. Waglerin-1-FAM is a valuable tool in fluorescence-based imaging studies, enabling detailed investigations into nAChR function, receptor localization, and the development of new therapeutic strategies for neuromuscular disorders.

Current Research:

Waglerin-1-FAM is a fluorescently labeled derivative of Waglerin-1, a 22-amino-acid peptide toxin isolated from the venom of Wagler's pit viper (Tropidolaemus wagleri). This conjugate combines the neuromuscular nicotinic acetylcholine receptor (nAChR) antagonistic properties of Waglerin-1 with the fluorescent capabilities of 6-carboxyfluorescein (FAM), facilitating the study of nAChR distribution and function.

Structural Characteristics

Waglerin-1-FAM retains the primary structure of Waglerin-1, with the addition of a FAM moiety covalently attached, typically at the N-terminus. This modification allows for fluorescence-based detection without significantly altering the peptide's biological activity.

Mechanism of Action

Similar to native Waglerin-1, Waglerin-1-FAM acts as a competitive antagonist of muscle-type nAChRs, particularly targeting receptors containing the ε subunit. By binding to the α/ε subunit interface, it inhibits acetylcholine-induced ion flux, thereby modulating synaptic transmission at the neuromuscular junction.

Applications in Research

The fluorescent labeling of Waglerin-1 enhances its utility in various research applications:

Receptor Localization: Waglerin-1-FAM enables visualization of nAChR distribution in tissues and cells, aiding in mapping receptor localization.

Binding Studies: The fluorescence tag facilitates quantitative analysis of peptide-receptor interactions, providing insights into binding kinetics and affinity.

Functional Assays: Waglerin-1-FAM can be used in assays to monitor receptor activity and investigate the effects of potential therapeutic agents on nAChR function.

Handling and Storage

Waglerin-1-FAM is typically supplied as a lyophilized powder. For optimal stability, it should be stored at -20°C, protected from light and moisture. Upon reconstitution, aliquots should be prepared to avoid repeated freeze-thaw cycles, which can degrade the peptide.

Conclusion

Waglerin-1-FAM serves as a valuable tool in neuropharmacological research, combining the specific nAChR antagonistic properties of Waglerin-1 with the advantages of fluorescent labeling. Its application facilitates the detailed study of nAChR distribution and function, contributing to advancements in understanding neuromuscular transmission and the development of targeted therapeutics.