390 MMP FRET Substrate 1

Product Name: 390 MMP FRET Substrate 1

Sequence One Letter Code: Mca-PLGL-Dap(Dnp)-AR-NH2

Sequence Three Letter Code: Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

Cas No: 140430-53-1

Chemical Formula:C49H68N14O15

Molecular Weight: 1093.2

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

SMILES: C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N)NC(=O)[C@H](C(C1=C(C=C(C=C1)[N+](=O)[O-])[N+](=O)[O-])NC(=O)[C@@H](CC(C)C)NC(=O)CNC(=O)[C@@H](CC(C)C)NC(=O)[C@H]2CCCN2C(=O)CC3=CC(=O)OC4=C3C=CC(=C4)OC)N.C(=O)(C(F)(F)F)O

IUPAC: (2R)-N-[(2R)-1-[[2-[[(2R)-1-[[(2S)-2-amino-3-[[(2S)-1-[[(2S)-1-amino-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-1-(2,4-dinitrophenyl)-3-oxopropyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-4-methyl-1-oxopentan-2-yl]-1-[2-(7-methoxy-2-oxochromen-4-yl)acetyl]pyrrolidine-2-carboxamide;2,2,2-trifluoroacetic acid

INCHIKEY: WOMSCEZBLCENEO-AGAGJJBFSA-N

INCHI:

InChI=1S/C49H68N14O15.C2HF3O2/c1-24(2)17-33(59-47(71)35-10-8-16-61(35)39(65)19-27-20-40(66)78-37-22-29(77-6)12-14-30(27)37)45(69)55-23-38(64)57-34(18-25(3)4)46(70)60-42(31-13-11-28(62(73)74)21-36(31)63(75)76)41(50)48(72)56-26(5)44(68)58-32(43(51)67)9-7-15-54-49(52)53;3-2(4,5)1(6)7/h11-14,20-22,24-26,32-35,41-42H,7-10,15-19,23,50H2,1-6H3,(H2,51,67)(H,55,69)(H,56,72)(H,57,64)(H,58,68)(H,59,71)(H,60,70)(H4,52,53,54);(H,6,7)/t26-,32-,33+,34+,35+,41-,42?;/m0./s1

Source / Species: Bovine

Conjugation: Conjugated

Conjugation Type: Double dyes

Code Nacres: NA.26

Application: MMP FRET Substrate 1 is a fluorogenic peptide substrate designed for sensitive detection of matrix metalloproteinase activity. It is preferentially cleaved by MMP-7, MMP-8, MMP-9, and MMP-13, producing a strong fluorescence increase after proteolytic cleavage at the Gly–Leu bond. The substrate contains a fluorescent donor and quencher pair, providing high signal-to-noise performance for enzyme activity assays. MMP FRET Substrate 1 is widely used to monitor metalloproteinase activity, protease kinetics, extracellular matrix remodeling, cancer invasion, inflammation, and tissue remodeling pathways. It is suitable for biochemical assays and high-throughput screening applications.