HIV Protease FRET Substrate I

Product Name: HIV Protease FRET Substrate I

Sequence One Letter Code: DABCYL-GABA-SQNYPIVQ-EDANS

Sequence Three Letter Code: DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS

Cas No: 127134-13-8

Chemical Formula:C73H97N17O18S

Molecular Weight: 1532.8

Purity: ≥ 95%

Form: Lyophilized

Storage Conditions: - 20 °C Protected from light

SMILES: CC[C@H](C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)NCCNC1=CC=CC2=C1C=CC=C2S(=O)(=O)O)NC(=O)[C@@H]3CCCN3C(=O)[C@H](CC4=CC=C(C=C4)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CO)NC(=O)CCCNC(=O)C5=CC=C(C=C5)N=NC6=CC=C(C=C6)N(C)C

IUPAC: 5-[2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S,3S)-2-[[(2S)-1-[(2S)-2-[[(2S)-4-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[4-[[4-[[4-(dimethylamino)phenyl]diazenyl]benzoyl]amino]butanoylamino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-4-oxobutanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]pyrrolidine-2-carbonyl]amino]-3-methylpentanoyl]amino]-3-methylbutanoyl]amino]-5-oxopentanoyl]amino]ethylamino]naphthalene-1-sulfonic acid

INCHIKEY: QWNZDVLUBHVXEG-OYZHKFNYSA-N

INCHI:

InChI=1S/C73H97N17O18S/c1-7-42(4)64(72(104)85-63(41(2)3)71(103)82-52(30-32-59(74)93)66(98)79-36-35-77-51-14-8-13-50-49(51)12-9-16-58(50)109(106,107)108)86-70(102)57-15-11-37-90(57)73(105)55(38-43-18-28-48(92)29-19-43)84-68(100)54(39-61(76)95)83-67(99)53(31-33-60(75)94)81-69(101)56(40-91)80-62(96)17-10-34-78-65(97)44-20-22-45(23-21-44)87-88-46-24-26-47(27-25-46)89(5)6/h8-9,12-14,16,18-29,41-42,52-57,63-64,77,91-92H,7,10-11,15,17,30-40H2,1-6H3,(H2,74,93)(H2,75,94)(H2,76,95)(H,78,97)(H,79,98)(H,80,96)(H,81,101)(H,82,103)(H,83,99)(H,84,100)(H,85,104)(H,86,102)(H,106,107,108)/t42-,52-,53-,54-,55-,56-,57-,63-,64-/m0/s1

Source / Species: HIV

Conjugation: Conjugated

Conjugation Type: Double dyes

Code Nacres: NA.26

Application: HIV Protease FRET Substrate I is a fluorogenic peptide substrate designed for continuous measurement of HIV-1 protease activity. Derived from a natural viral polyprotein cleavage site, this substrate is specifically hydrolyzed at the Tyr–Pro bond, generating a time-dependent fluorescence increase. HIV-1 protease is essential for viral maturation and remains a major therapeutic target in AIDS drug development. HIV Protease FRET Substrate I enables sensitive kinetic analysis, real-time enzymatic monitoring, and inhibitor screening. It is widely used in antiviral research, protease mechanism studies, drug discovery, and assay development.