Histone H3 (1-34)

Product Name:Histone H3 (1-34)

Purity:95%

Molar Mass:3451.98

Chemical Formula:C144H260N54O44

Storage:Store at -20 degrees Celsius

Sequence:ARTKQTARKSTGGKAPRKQLATKAARKSAPATGG

Application:Histone H3 (1-34) is a peptide corresponding to the first 34 amino acids of the histone H3 protein. This region of histone H3 is highly conserved and plays a crucial role in the regulation of chromatin structure and gene expression. It includes key sites for post-translational modifications such as methylation, acetylation, and phosphorylation, which are essential for regulating DNA accessibility and transcriptional activity. Researchers use Histone H3 (1-34) to study chromatin dynamics, histone modifications, and their impacts on epigenetic regulation, gene expression, and processes such as cell cycle control, development, and cancer. This peptide is integral to advancing our understanding of epigenetic mechanisms.

Current Research:

Histone H3 (1-34) is a synthetic peptide corresponding to the first 34 amino acids of the N-terminal tail of histone H3, a core component of the nucleosome in eukaryotic chromatin. This region is subject to various post-translational modifications (PTMs) that play crucial roles in regulating chromatin structure and gene expression.
Structural Features
The N-terminal tail of histone H3 is rich in lysine and arginine residues, which are common sites for PTMs such as methylation, acetylation, and phosphorylation. These modifications can influence chromatin dynamics by altering histone-DNA interactions and serving as binding sites for effector proteins.
Biological Significance
Modifications within the H3 (1-34) region are integral to the "histone code," a hypothesis suggesting that specific PTM patterns on histones can recruit distinct protein complexes, thereby influencing transcriptional outcomes. For example, methylation of lysine 4 (H3K4me) is generally associated with active transcription, while methylation of lysine 27 (H3K27me) is linked to gene repression.
Research Applications
The histone H3 (1-34) peptide is widely used in biochemical assays to study histone interactions, enzymatic activities, and the effects of specific PTMs. It serves as a substrate for histone-modifying enzymes, allowing researchers to investigate the mechanisms of histone modification and their impact on chromatin function.
Conclusion
As a representative segment of histone H3, the (1-34) peptide is a valuable tool in epigenetic research, providing insights into the complex regulation of gene expression mediated by histone modifications.

Reference:

Zheng, S., Bi, Y., Chen, H., Gong, B., Jia, S., & Li, H. (2021). Molecular basis for bipartite recognition of histone H3 by the PZP domain of PHF14. Nucleic acids research, 49(15), 8961-8973.