VKGILS-NH2

Product Name:VKGILS-NH2

CAS No:942413-05-0

Purity:95%

Molar Mass:614.79

Chemical Formula:C28H54N8O7

Storage:Store at -20 degrees Celsius

Sequence:VKGILS

Target:PAR2

Application:VKGILS-NH2 is a synthetic peptide consisting of the amino acid sequence Valine-Lysine-Glycine-Isoleucine-Leucine-Serine, with an amidated C-terminus (NH2). This peptide is used in research for its potential role in modulating biological processes such as protein interactions and cell signaling pathways. It may serve as a ligand or inhibitor in studies focused on understanding specific receptor functions or cellular responses. The amidation of the C-terminus can enhance peptide stability and receptor binding affinity, making it useful for experimental applications in peptide-receptor studies and biochemical assays.

Current Research:

VKGILS-NH₂ is a synthetic hexapeptide (Val-Lys-Gly-Ile-Leu-Ser) with a C-terminal amide group. This peptide functions as a reversed-sequence control for SLIGKV-NH₂, a well-characterized agonist of protease-activated receptor 2 (PAR₂). Due to its reversed sequence, VKGILS-NH₂ does not activate PAR₂ and is biologically inert in the context of PAR₂ signaling, making it an essential tool in receptor pharmacology research.

Biological Properties

In studies involving PAR₂ activation, VKGILS-NH₂ demonstrates no significant impact on intracellular signaling pathways such as calcium mobilization or DNA synthesis. This inert nature allows researchers to confirm the specificity of SLIGKV-NH₂-induced effects by comparing responses elicited by the agonist and its reversed-sequence control. By ensuring that observed effects are receptor-specific, VKGILS-NH₂ enhances the reliability of experimental findings.

Applications in Research

VKGILS-NH₂ is widely used to validate PAR₂-mediated biological processes. Its primary applications include:

Control Peptide in PAR₂ Studies: VKGILS-NH₂ serves as a negative control, ensuring that experimental outcomes are attributable to SLIGKV-NH₂ activation of PAR₂ rather than nonspecific interactions.
Cellular Response Validation: It provides a baseline for distinguishing between specific receptor activation effects and general cellular responses.
Drug Discovery and Validation: VKGILS-NH₂ helps in testing the specificity of novel PAR₂-targeted therapeutics by serving as a reference in comparative assays.
Significance in Receptor Pharmacology

The specificity and inert nature of VKGILS-NH₂ make it indispensable in research focusing on PAR₂, a receptor implicated in inflammation, pain, and vascular homeostasis. By eliminating experimental ambiguities, VKGILS-NH₂ contributes to advancing our understanding of PAR₂ signaling pathways and their therapeutic potential.

Conclusion

VKGILS-NH₂ is a critical tool for ensuring specificity in PAR₂-related research. Its role in validating receptor-mediated effects supports accurate interpretation of experimental results, ultimately facilitating advancements in receptor biology and pharmacological interventions.

Reference:

Kim, M. S., Jo, H., Um, J. Y., Yi, J. M., Kim, D. K., Choi, S. C., … & Lee, Y. M. (2002). Agonists of proteinase‐activated receptor 2 induce TNF‐αsecretion from astrocytoma cells. Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease, 20(4), 339-345.