[Lys(Me1)9]-Histone H3 (3-17)

Product Name: [Lys(Me1)9]-Histone H3 (3-17)

Sequence One Letter Code: TKQTAR-K(Me1)-STGGKAPR

Sequence Three Letter Code: H-Thr-Lys-Gln-Thr-Ala-Arg-Lys(Me1)-Ser-Thr-Gly-Gly-Lys-Ala-Pro-Arg-OH

Molecular Weight: 1600.9

Purity: 95%

Form: Lyophilized

Storage Conditions: - 20 °C

Research Area: epigenetics

Source / Species: human

Conjugation: Unconjugated

Code Nacres: NA.26

Application: [Lys(Me1)9]-Histone H3 (3–17) is a synthetic peptide representing histone H3 residues 3–17 with monomethylation at lysine 9 (H3K9me1). This modification is a dynamic epigenetic mark involved in chromatin state regulation and transcriptional control. H3K9me1 often acts as a precursor for higher methylation states and participates in both active and repressive chromatin contexts. It plays a role in chromatin remodeling and gene expression transitions. This peptide is widely used to study histone methylation pathways, enzyme specificity, and the functional progression of lysine methylation. It is suitable for binding assays, enzymatic studies, and epigenetic research focused on chromatin regulation and modification dynamics.

Current Research: Histone methylation is a key regulatory mechanism in chromatin biology, with different methylation states of lysine residues contributing to distinct functional outcomes. Monomethylation of lysine 9 on histone H3 (H3K9me1) represents an important and dynamic epigenetic mark involved in chromatin state transitions and gene regulation. [Lys(Me1)9]-Histone H3 (3–17) is a synthetic peptide designed to replicate this modification within a defined segment of the histone H3 N-terminal region, providing a valuable tool for investigating histone methylation pathways and chromatin dynamics. H3K9 Monomethylation in Chromatin Regulation H3K9 methylation exists in three forms—mono-, di-, and trimethylation—each associated with different chromatin states. H3K9me1 occupies a unique position within this spectrum, often functioning as an intermediate or precursor modification that can be further converted into di- or trimethylated states (H3K9me2 and H3K9me3), which are typically linked to transcriptional repression. Unlike higher methylation states that are strongly associated with heterochromatin, H3K9me1 is more functionally flexible and can be found in both active and repressive chromatin contexts. It is frequently involved in: Chromatin remodeling and structural transitions Regulation of gene expression during dynamic cellular processes Establishment of methylation states that define chromatin identity This versatility makes H3K9me1 an important mark for studying how chromatin states evolve over time. Structural Design of the Peptide The [Lys(Me1)9]-Histone H3 (3–17) peptide corresponds to amino acids 3–17 of histone H3, a region within the N-terminal tail that is highly accessible and subject to multiple regulatory modifications. In this synthetic construct, lysine at position 9 is monomethylated, accurately reflecting the H3K9me1 modification found in vivo. By isolating this single modification within a short peptide sequence, researchers can examine its specific effects on protein binding and enzymatic activity without interference from other histone marks. This design makes the peptide particularly suitable for controlled biochemical and mechanistic studies. Role as a Precursor in Methylation Pathways One of the defining features of H3K9me1 is its role as a substrate for further methylation. Histone methyltransferases can convert H3K9me1 into H3K9me2 and H3K9me3, which are associated with progressively more stable repressive chromatin states. Studying H3K9me1 provides insight into: Enzyme specificity and catalytic mechanisms of histone methyltransferases Sequential methylation processes in chromatin regulation Dynamic transitions between active and repressive chromatin states By using this peptide, researchers can dissect how methylation states are established and maintained within the genome. Applications in Epigenetics and Enzymatic Studies Because it represents an early stage in histone methylation, [Lys(Me1)9]-Histone H3 (3–17) is widely used in studies focused on epigenetic modification pathways and enzyme function. Common applications include: Histone methyltransferase assays to evaluate enzyme activity and specificity Substrate studies examining conversion to higher methylation states Protein–peptide interaction assays for identifying H3K9me1-binding proteins Biochemical studies of chromatin remodeling processes Investigation of epigenetic signaling pathways These applications help clarify how histone methylation contributes to chromatin regulation and gene expression. Role in Gene Expression and Chromatin Dynamics H3K9me1 is particularly relevant in contexts where chromatin states are actively changing, such as during development, differentiation, or cellular response to environmental signals. Its presence can indicate regions undergoing epigenetic remodeling, where genes may be transitioning between active and repressed states. This dynamic behavior distinguishes H3K9me1 from more stable repressive marks and highlights its importance in fine-tuning gene expression programs. A Valuable Tool for Studying Methylation Progression Synthetic histone peptides such as [Lys(Me1)9]-Histone H3 (3–17) provide a precise platform for analyzing the stepwise progression of histone methylation. By replicating the monomethylated state of H3K9, this peptide enables detailed investigation of how chromatin modifications evolve and how they influence transcriptional outcomes. Through its use in enzyme assays, binding studies, and chromatin research, this peptide supports a deeper understanding of epigenetic regulation, histone modification dynamics, and the mechanisms that govern gene expression and chromatin structure.