VSV-G Peptide

Product Name: VSV-G Peptide

Sequence One Letter Code: YTDIEMNRLGK

Sequence Three Letter Code: H-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-OH

Chemical Formula:C57H94N16O19S

Molecular Weight: 1339.5

Purity: 95%

Form: Lyophilized

Storage Conditions: - 20 °C

Research Area: Antiviral

Source / Species: VSV

Conjugation: Unconjugated

Code Nacres: NA.26

Application: VSV-G Peptide is a synthetic fragment derived from the vesicular stomatitis virus glycoprotein G, a viral envelope protein critical for host cell attachment and membrane fusion. VSV-G mediates broad cellular tropism and is widely utilized in viral pseudotyping and gene delivery systems. Peptides derived from this protein are used to investigate virus–host membrane interactions, fusion mechanisms, and receptor engagement processes. In addition to virology research, VSV-G–based peptides support antibody characterization, vaccine epitope mapping, and studies of viral antigen recognition. This peptide provides a defined model for analyzing viral entry pathways and membrane fusion dynamics in controlled experimental systems.

Current Research: VSV-G Peptide is a synthetic fragment derived from the vesicular stomatitis virus (VSV) glycoprotein G, a viral envelope protein essential for host cell attachment, endocytosis, and membrane fusion. VSV-G is responsible for the broad cellular tropism of VSV and is widely used in viral pseudotyping platforms due to its ability to mediate efficient entry into diverse mammalian cell types. Synthetic peptides derived from this glycoprotein provide defined tools for studying virus–host interactions, membrane fusion mechanisms, and antigenic recognition in controlled experimental systems. Biological Role of VSV-G The VSV glycoprotein G is a class III viral fusion protein embedded in the viral envelope. It mediates viral entry through receptor engagement followed by endocytic uptake and pH-dependent conformational changes that drive membrane fusion within endosomes. Upon acidification, VSV-G undergoes structural rearrangements that expose fusion loops, facilitating insertion into the host membrane and subsequent bilayer merger. Because of its robust fusogenic properties and broad host range, VSV-G is commonly used to pseudotype lentiviral and retroviral vectors, enhancing their infectivity across multiple cell types. Peptide fragments derived from VSV-G allow detailed dissection of functional domains involved in receptor binding and membrane destabilization. Applications in Viral Entry and Fusion Research VSV-G–based peptides are employed to investigate the molecular mechanisms underlying viral membrane fusion. Specific domains within the glycoprotein are associated with receptor interaction, conformational rearrangement, and lipid bilayer destabilization. Synthetic peptides corresponding to these regions support: Analysis of virus–host membrane interaction Characterization of fusion loop activity Evaluation of pH-dependent conformational changes Competitive inhibition assays targeting viral entry These studies contribute to broader understanding of viral entry strategies across enveloped viruses. Utility in Pseudotyping and Gene Delivery Systems In gene therapy and viral vector engineering, VSV-G pseudotyping is widely used to enhance vector stability and expand host cell tropism. Peptides derived from VSV-G facilitate mechanistic evaluation of envelope–receptor interactions and help optimize vector design. They are also applied in experiments aimed at modulating or blocking viral entry in transduction assays. Antibody Characterization and Epitope Mapping Beyond fusion research, VSV-G peptides serve as defined antigenic fragments for antibody characterization. Because VSV-G is frequently incorporated into viral vector systems, detection and quantification of VSV-G expression are important in manufacturing and quality control workflows. Applications include: Mapping of neutralizing antibody epitopes ELISA-based antibody detection assays Surface plasmon resonance (SPR) binding studies Vaccine antigen recognition analysis Defined peptide fragments allow precise identification of antibody-binding determinants and facilitate evaluation of immune responses targeting VSV-G. Broader Research Applications VSV-G Peptide is also utilized in: Structural studies of viral fusion domains Membrane biophysics investigations Liposome interaction assays Comparative analysis of class III viral fusion proteins Because the peptide represents a specific region of a well-characterized viral envelope protein, it provides a reproducible model for studying viral membrane dynamics without requiring intact viral particles. Experimental Advantages Derived from a well-characterized viral fusion protein Supports mechanistic analysis of membrane fusion Applicable in viral vector and gene delivery research Useful for antibody and epitope mapping studies Suitable for biochemical and cell-based assays Research Significance VSV-G Peptide serves as a versatile tool in virology, gene delivery, and immunological research. By enabling controlled examination of viral glycoprotein–mediated membrane interactions, it supports detailed investigation of viral entry pathways, receptor engagement, and antigen recognition. Its defined sequence and functional relevance make it valuable in both fundamental viral biology studies and translational applications involving viral vector systems.